piRNA DQ725559 Resists Myocardial Ischemia-reperfusion Injury by Inhibiting the Ferroptosis Pathway of Cardiomyocytes
To explore the relationship between myocardial ischemia-reperfusion injury(IRI)and ferropto-sis,and to find related Piwi-interacting RNA(piRNA),myocardial cell IRI model was constructed by Hy-poxia/Reoxygenation(H/R)of primary cardiomyocytes from neonatal mice.The optimal H/R time point for inducing ferroptosis was reflected by cell survival rate.piRNA associated with ferroptosis was screened and changes of ferroptosis related indexes were detected.The results show that ferroptosis is associated with myocardial IRI after hypoxia for 18 h and reoxygenation for 6 h.Inhibition of DQ725559 expression aggravates ferroptosis and myocardial IRI,while overexpression of DQ725559 alleviates ferroptosis and myocardial IRI.This suggests that DQ725559 can play a role in the regulation of myocardial IRI by regula-ting the ferroptosis pathway of cardiomyocytes,and overexpression of DQ725559 could be a new strategy for RNA therapy of cardiovascular diseases.
国家科技期刊平台
NSTL
万方数据
维普
