Mechanistic Study of Isatin Against Oligoasthenozoospermia Based on Network Pharmacology and Molecular Docking
To investigate the potential targets and mechanisms of isatin(ISA)in the treatment of oligoas-thenozoospermia,targets of ISA and disease-related targets were identified utilizing public databases to find intersecting targets.Core targets were then determined using Cytoscape software.GO functional en-richment and KEGG pathway analyses on the intersecting targets were conducted,and molecular docking was used to predict the binding affinity of ISA with core target proteins.The results indicate that ISA's in-tervention in oligoasthenozoospermia primarily involves biological processes such as oxidative stress,apop-tosis,and inflammation,and is closely related to the p53 signaling pathway,the cell senescence pathway,and the IL-17 signaling pathway.After screening,eight core targets were identified,with ISA stably bind-ing to six of them.Those suggest that ISA may exert its anti-oligoasthenospermia effects by modulating the p53 signaling pathway and the IL-17 signaling pathway through core targets including CASP3,TP53,ESR1,PTGS2,TNF and ANXA5.
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