首页|红嘴鸥和喜鹊的粪便微生物群多样性比较研究

红嘴鸥和喜鹊的粪便微生物群多样性比较研究

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为评估不同种鸟类的病原体传播风险,提取红嘴鸥和喜鹊粪便的总DNA,通过PCR扩增16S rRNA序列V3~V4高变区,基于高通量测序和生物信息学研究两者肠道微生物群差异.研究结果显示,红嘴鸥和喜鹊粪便微生物群组成存在显著差异,两者粪便中最丰富的细菌门分别是厚壁菌门和变形菌门,前者粪便中绝对优势属是Catellicoccus(丰度均值65.5%).红嘴鸥样本中均检测出弯曲杆菌属,检出率100%,丰度均值1.2%;喜鹊样本检出率55.6%,丰度均值0.019%.这表明,青岛市红嘴鸥种群广泛存在弯曲杆菌属,红嘴鸥的接触安全风险高于喜鹊.
Comparative Study on the Fecal Microbiota Diversity of Black-Headed Gull and Magpie
Total DNA from fecal samples were extracted and the V3~V4 hypervariable region of the 16S rRNA sequence was amplified to assess the potential risk of bird-to-human pathogen transmission in differ-ent bird species.Gut microbiota characterizations were determined with high-throughput sequencing and the followed bioinformatical analyses.The results show remarkable differences in the fecal microbiota be-tween black-headed gulls and magpies.Firmicutes and Proteobacteria are the primary bacterial phyla in black-headed gull and magpie feces,respectively.Catellicoccus is the predominant genus in black-headed gull feces,and the average abundance is 65.5%.The detective rate of Campylobacter in black-headed gull feces is 100%,and the average abundance is 1.2%.But in magpie feces,the detective rate is 55.6%,and the average abundance is 0.019%.This suggests that Campylobacter is widespread in the population of black-headed gull in Qingdao,and the safety risk of exposure to black-headed gulls is higher than magpies.

black-headed gullmagpieintestinal microbiotahigh-throughput sequencing

刘文轩、杨笑礼、李宗会、王斌

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青岛大学基础医学院,青岛 266000

红嘴鸥 喜鹊 肠道菌群 高通量测序

2024

青岛大学学报(自然科学版)
青岛大学

青岛大学学报(自然科学版)

影响因子:0.248
ISSN:1006-1037
年,卷(期):2024.37(3)