Comparative Study on the Fecal Microbiota Diversity of Black-Headed Gull and Magpie
Total DNA from fecal samples were extracted and the V3~V4 hypervariable region of the 16S rRNA sequence was amplified to assess the potential risk of bird-to-human pathogen transmission in differ-ent bird species.Gut microbiota characterizations were determined with high-throughput sequencing and the followed bioinformatical analyses.The results show remarkable differences in the fecal microbiota be-tween black-headed gulls and magpies.Firmicutes and Proteobacteria are the primary bacterial phyla in black-headed gull and magpie feces,respectively.Catellicoccus is the predominant genus in black-headed gull feces,and the average abundance is 65.5%.The detective rate of Campylobacter in black-headed gull feces is 100%,and the average abundance is 1.2%.But in magpie feces,the detective rate is 55.6%,and the average abundance is 0.019%.This suggests that Campylobacter is widespread in the population of black-headed gull in Qingdao,and the safety risk of exposure to black-headed gulls is higher than magpies.