首页|Novel Functions of Interleukin-1 Beta in Spotted Halibut (Verasper variegatus) at Its Embryonic and Metamorphic Stages

Novel Functions of Interleukin-1 Beta in Spotted Halibut (Verasper variegatus) at Its Embryonic and Metamorphic Stages

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Interleukin-1beta(IL-1β)is a cytokine involved in the control of inflammatory responses.The functions of IL-1β have been extensively studied in many vertebrates.However,few studies focused on the functions of IL-1β in the spotted halibut(Verasper variegatus),an endangered aquatic fish that easily suffers from bacterial and viral infections.We first cloned and sequenced the IL-1β gene from V.variegatus(VvIL-1β).Its conservative interleukin-1 propeptide and interleukin-1/18 domains corresponding regions,characteristics of the IL-1 protein family and TA-rich motifs relating to inflammatory responses were similar to those of the IL-1β genes from other teleost and mammals.The phylogenetic analysis showed that VvIL-1β was homologous to the IL-1β gene of other vertebrates.Treatment with pathogen-associated molecular patterns(PAMPs)induced a significant and rapid up-regulation of VvIL-1β expression in the peripheral blood leukocytes(PBLs),revealing that VvIL-1β is actively involved in the host immune responses against bacterial and viral pathogens.The expression level of VvIL-1β was relatively high in the PBLs,gill,and intestine,indicating the immunological function of VvIL-1β.VvIL-1β also had interesting expression patterns at specific developmental stages,implying that the IL-1β of flatfishes may have some novel functions during embryonic development and metamorphosis.The 3D structure prediction also provided information about how VvIL-1β interacts with its receptor protein.These results suggested that VvIL-1β may have multiple functions in V.variegatus and provided us valuable information for understanding the novel functions of IL-1β.

IL-1βVerasper variegatusexpression patternmetamorphosisperipheral blood leukocyte3D modelingpathogen-associated molecular pattern

LI Zan、LIU Xiumei、CAO Dandan、LIU Yuezhong、WANG Xubo、LIU Jinxiang、WANG Zhigang、ZHANG Quanqi、YU Haiyang

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School of Agriculture,Ludong University,Yantai 264025,China

Key Laboratory of Marine Genetics and Breeding of Ministry of Education,College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China

This research was supported by the National Natural Sciences Foundation of Chinaand the Hitech Research and Development Program of China

311018912012AA10A408

2018

中国海洋大学学报(自然科学英文版)
中国海洋大学

中国海洋大学学报(自然科学英文版)

CSTPCDCSCDSCI
影响因子：0.268
ISSN：1672-5182
年,卷(期)：2018.17(5)
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