首页|Analysis of DNA Methylation Level of Portunus trituberculatus Subjected to Low Salinity with Methylation-Sensitive Amplification Polymorphism

Analysis of DNA Methylation Level of Portunus trituberculatus Subjected to Low Salinity with Methylation-Sensitive Amplification Polymorphism

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
In this study,the methylation-sensitive amplification polymorphism (MSAP) was used to compare the genomic DNA methylation level of muscle,gill and hepatopancreas of Portunus trituberculatus subjected to salinity 12 for 30 days to illustrate the epigenetic mechanism of osmoregulation.Thirty primers were used to analyze the difference of methylation level of different tissues.The results showed that the baseline methylation level of muscle,hepatopancreas and gill was 47.31％,22.94％ and 17.69％,respec tively.After exposed to low salinity stress,the methylation epiloci changed in the three tissues.Both demethylation and methylation processes occurred under low salinity stress.The methylation ratio decreased in muscle and gill but increased in hepatopancreas.These results indicated that DNA methylation is tissue-specific when P.trituberculatus responds to low salinity.

Portunus trituberculatusDNA methylationmethylation-sensitive amplification polymorphismlow salinitygillmusclehepatopancreas

LU Shaokun、LI Ronghua、GAO Tianlong、WANG Chunlin、ZHENG Weibing、MU Changkao、SONG Weiwei

展开 >

Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China

Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy ,4quaculture, Ningbo University, Ningbo 315211, China

Third Institute of Oceanography, State Oceanic Administration, Xiamen 361001, China

This study was supported by the grants from the National Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang ProvinceMajor Agriculture Program of NingboNingbo Science and Technology ProjectOpen Fund of Ningbo UniversityOpen Fund of Ningbo University

No.4147 6124No.LY17C190005No.2017C110007No.2016C10037No.xkzsc1505K C Wong Magana Fund in Ningbo University.We would like to thank Dr.Sarah-Louise C

2019

中国海洋大学学报(自然科学英文版)
中国海洋大学

中国海洋大学学报(自然科学英文版)

CSTPCDCSCDSCI
影响因子：0.268
ISSN：1672-5182
年,卷(期)：2019.18(5)
  • 5