首页|Expression and Characterization of a Novel λ-Carrageenase Cgl150A_Wa from Wenyingzhuangia aestuarii
Expression and Characterization of a Novel λ-Carrageenase Cgl150A_Wa from Wenyingzhuangia aestuarii
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
λ-Carrageenan is a highly sulfated polysaccharide alternating of 1,4-O-α-D-galactopyranose-2,6-sulfate(D2S,6S)and 1,3-O-β-D-galactopyranose-2-sulfate(G2S).λ-Carrageenases are desirable tools for λ-carrageenan degradation.Based on the genome mi-ning,a novel λ-carrageenase Cgl150A_Wa was cloned from the bacterium Wenyingzhuangia aestuarii and expressed in Escherichia coli.Cgl150A_Wa was an endo-acting enzyme and exhibited its maximum activity at 30℃and pH 8.0.By employing a glycomics strategy that combined ultra-performance liquid chromatography-mass spectrometry analysis and glycoinformatics,Cgl150A_Wa was proven to degrade λ-carrageenan octaose and hexaose,and the major hydrolysis product of Cgl150A_Wa was λ-carrageenan tetrose.In addition to the typical λ-carrageenan motifs,the active center of Cgl150A_Wa might tolerate desulfated λ-carrageenan motifs.Cgl150A_Wa is a potential biotechnological tool for preparing λ-carrageenan oligosaccharides and structural investigation.
NETL
NSTL
万方数据
