首页|Characterization of a Novel Esterase Belonging to Family V from Marinobacter flavimaris
Characterization of a Novel Esterase Belonging to Family V from Marinobacter flavimaris
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Lipolytic enzymes have attracted enormous attentions because of their ability in ester hydrolysis,ester synthesis,trans-esterification and other biochemical reactions.Bacteria are important sources of lipolytic enzymes applied in industry.Here,a novel lipolytic enzyme encoded by esterase gene est1347 was identified in Marinobacter flavimaris WLL162,and was purified and cha-racterized.The lipolytic enzyme Est1347 consisted of 312 amino acid residues and a 21-amino-acids N-terminal signal peptide with a predicted molecular weight of 34.2 kDa.It belongs to family V of bacterial lipolytic enzymes based on the amino acid sequence ho-mology analysis.Est1347 is a mesophilic and alkali-resistant enzyme with the highest activity at 45℃and pH 8.5;it is stable at tem-peratures below 50℃and pH 7.5-11.0.Est1347 showed a preference for middle-length chain substrate p-NPC10 and a wide range of other substrates.The Km,Vmax,Kcat and Kcat/Km values of Est1347 for p-NPC10 in pH 8.5 at 45℃were 0.9411 mmol L-1,1285 μmol min-1 mg-1,698.91 s-1 and 743.65 s-1(mmol L-1)-1,respectively.It is also tolerant to the metal ions,organic solvents and deter-gents.In conclusion,the esterase Est1347 laid a foundation for further study of bacterial lipolytic enzyme family V.
esteraseMarinobacter flavimarisenzymatic propertieslipolytic enzyme family V
HE Jingjing、ZHANG Yunhui、WU Leilei、WANG Yaru、ZHANG He、LIU Zhengang、SHI Xiaochong
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Frontiers Science Center for Deep Ocean Multispheres and Earth System,and College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China
Laboratory for Marine Ecology and Environmental Science,Laoshan Laboratory,Qingdao 266237,China
Institute of Evolution & Marine Biodiversity,Ocean University of China,Qingdao 266003,China
projects from the National Natural Science Foundation of ChinaChina Ocean Mineral Resources R and D Association(COMRA)Special Foundation
NETL
NSTL
万方数据
维普
