首页|Molecular Characterization and Antibacterial Activity of a G-Type Lysozyme in Yellow Drum(Nibea albiflora)

Molecular Characterization and Antibacterial Activity of a G-Type Lysozyme in Yellow Drum(Nibea albiflora)

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Lysozyme(EC3.2.1.17)plays an important role in the immune response;as a nonspecific immune factor,it can resist cau-sative agents.Lysozyme can be divided into c-type and g-type in fish.In a previous study,through genome-wide association analysis,the g-type lysozyme gene,which is named NaLyg in yellow drum(Nibea albiflora),was found to be a key candidate gene for disease resistance in response to Vibrio harveyi infection.The cDNA of NaLyg was 1025 bp,including four exons and three introns,and its open reading frame(ORF)had a full-length of 582bp,encoding 193 amino acids.NaLyg was found to be conserved during evolution through bioinformatic analyses.The NaLyg protein possessed a sugar binding domain and three catalytic sites,including Glu71,Asp84 and Asp101.Quantitative qRT-PCR results confirmed that NaLyg gene mRNA was visibly increased after V.harveyi infection.The NaLyg protein purified by prokaryotic expression killed some gram-negative bacterial pathogens by inducing cell wall destruction,including V.harveyi,Aeromonas hydrophila and Edwardsiella tarda.Moreover,the NaLyg protein killed two gram-positive bacteria,Bacillus subtilis and Staphylococcus aureus.Taken together,the experimental results suggested that the NaLyg protein of N.albiflo-ra played an important role in fighting bacterial infections.

Nibea albiflorainnate immunityVibrio harveyig-type lysozymeantibacterial activity

XIAO Yao、LI Jiacheng、TIAN Qianqian、WU Baolan、HAN Fang

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State Key Laboratory of Mariculture Breeding,Fisheries College of Jimei University,Xiamen 361021,China

National Natural science Foundation of ChinaNational Key R&D Program of ChinaNatural Science Foundation of Fujian ProvinceOpen Research Fund Program of Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment

320729692022YFD24010022022 J01325Z822280

2024

10.1007/s11802-024-5697-7

中国海洋大学学报(自然科学英文版)
中国海洋大学

中国海洋大学学报(自然科学英文版)

CSTPCD
影响因子：0.268
ISSN：1672-5182
年,卷(期)：2024.23(2)
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