首页|Identification and Expression Profile of a Neuropeptide LFRFamide-Like Gene During Different Stages of Gonadal Development in the Cephalopod Sepia pharaonis

Identification and Expression Profile of a Neuropeptide LFRFamide-Like Gene During Different Stages of Gonadal Development in the Cephalopod Sepia pharaonis

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Neuropeptides are widely distributed in vertebrates and invertebrates,regulating a variety of physiological activities in the organisms,such as metabolism,feeding and reproduction.In this study,to explore the function of neuropeptide LFRFamide in Sepia pharaonis,the full-length cDNA of LFRFamide-like gene(named SpLFRFL,MG869822.1)was identified with rapid amplification of cDNA ends(RACE)method.The sequence of SpLFRFL was 860 bp in length and encoded 188 amino acids containing 4 different ma-ture peptides:1 copy of PHTPFRFamide,NSLFRFamide,TIFRFamide,and 3 copies of GNLFRFamide.Multiple alignment and phy-logenetic analysis results showed that SpLFRFL shared high identity with LFRFamides of Sepia officinalis and Sepiella japonica and had the closest relationship with them.Through quantitative Real-time PCR(qRT-PCR),it was found that the SpLFRFL gene was high-ly expressed in the optic lobe and brain at three different stages during gonad development in both genders.Moreover,the four ma-ture peptides at a concentration of 0.01 μmol L-1 could inhibit the protein synthesis in the Chinese hamster ovary cell strain-K1(CHO-Kl)induced by SpGnRH.These data suggest that SpLFRFL might be involved in the development and reproduction of SS.pharaonis.The results can contribute to future studies on neuropeptide evolution and function and benefit the cuttlefish farming.

neuropeptideLFRFamide-likecephalopodcuttlefishSepia pharaonisdevelopmentreproduction

QIU Jiayin、SUN Lianlian、LI Shuang、ZHOU Xu、CHI Changfeng、ZHENG Libing

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National and Provincial Joint Engineering Research Center for Marine Germplasm Resources Exploration and Utilization,School of Marine Science and Technology,Zhejiang Ocean University,Zhoushan 316022,China

National Natural science Foundation of ChinaNatural science Foundation of Zhejiang Province,ChinaOpen Foundation from Marine Sciences in the First-Class Subjects of ZhejiangExcellent Postdoctoral Program of Jiangsu Province

31872547LY20 C190007OFMS004314865

2024

10.1007/s11802-024-5715-9

中国海洋大学学报(自然科学英文版)
中国海洋大学

中国海洋大学学报(自然科学英文版)

CSTPCD
影响因子：0.268
ISSN：1672-5182
年,卷(期)：2024.23(2)
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