首页|Identification,Characterization,and Probiotic Potentials of Lactobacillus pentosus SF-1

Identification,Characterization,and Probiotic Potentials of Lactobacillus pentosus SF-1

扫码查看
原文链接
  • 万方数据
  • 维普
In recent years probiotics have been considered as a potential substitution of antibiotics to control pathogens and treat in-fectious diseases in aquaculture.In the present study a strain of Lactobacillus pentosus,named as L.pentosus SF-1,was isolated from waters in aquaculture.The species identification of this strain was conducted by 16S rRNA sequence,and the physiological and bio-chemical characteristics of this strain were assessed.Furthermore,the virulence,antibiotic sensitivity,cell surface characteristics and acid/base-resistance of L.pentosus SF-1 were determined to evaluate the probiotic potentials of this strain.Specifically,L.pentosus SF-1 is sensitive to most common antibiotics,and no hemolysin was generated from it,indicating the safety of this strain to hosts.In addition,L.pentosus SF-1 was able to tolerate the artificial gastric juice at pH 3 for 4h and the artificial intestinal fluid at pH 6.8 or 8.0 for 6h.Moreover,the analysis of self-aggregation and the adhesion of L.pentosus SF-1 to organic solvents suggested a high po-tential of L.pentosus SF-1 to inhabit the hosts,which was confirmed by testing the colonization of L.pentosus SF-1 in germ-free zeb-rafish.Interestingly,L.pentosus SF-1 displayed a high bactericidal activity against several bacterial pathogens.Consistently,the incu-bation of L.pentosus SF-1 significantly promoted the expression of antimicrobial components in zebrafish,contributing to the protec-tion of the fish from E.tarda infection in vivo.Taken together,the probiotic strain L.pentosus SF-1 could be applied as anti-infection reagent in aquaculture.

Lactobacillus pentosusprobioticsantibioticshemolysiscell surface characteristicsbactericidal activityinfection

JIAO Lin、LIANG Shufei、CHU Ruixia、WANG Wentao、MAI Kangsen、LIU Ming、WAN Min

展开 >

Key Laboratory of Aquaculture Nutrition and Feed,Ministry of Agriculture & Key Laboratory of Mariculture,Ministry of Education,College of Fisheries,Ocean University of China,Qingdao 266003,China

Engineering Research Center of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China

Key Laboratory of Microbiology of Shandong University for their technical supports for the HPLC detectionNational Natural Science Foundation of ChinaNatural Science Foundation of Shandong ProvinceTaishan Scholar Program of Shandong Province

31972802ZR2019MC041tsqn201812023

2024

10.1007/s11802-024-5716-8

中国海洋大学学报(自然科学英文版)
中国海洋大学

中国海洋大学学报(自然科学英文版)

CSTPCD
影响因子：0.268
ISSN：1672-5182
年,卷(期)：2024.23(2)
  • 40