首页|Endogenous Trypsin Triggers Free Fluoride Release from Antarctic Krill(Euphausia superba)Cuticle

Endogenous Trypsin Triggers Free Fluoride Release from Antarctic Krill(Euphausia superba)Cuticle

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During postmortem storage,fluoride in Antarctic krill can be enriched in the muscle.Trypsin,as the most important di-gestive enzyme in Antarctic krill with a high activity in low temperature,plays a potential role in this process.In this study,endo-genous trypsin was purified and its properties were investigated.The involvement of trypsin in the generation of free fluoride from Antarctic krill cuticle was explored.Cuticle microstructure before and after hydrolysis was compared with scanning electron micro-scopy,and the ash samples of the hydrolyzed Antarctic krill cuticle were analyzed with X-ray diffraction,Fourier transform infrared spectroscopy,and electron dispersive spectroscopy,respectively.Mass spectrometry analysis and inhibition tests confirmed that the purified enzyme was endogenous trypsin.Results of the present study indicated that trypsin digestion caused the increases of the concentrations of both fluoride ions and free amino N simultaneously,while the protein coated on the cuticle surface was dissolved too.However,no compositional change was detected in thecuticle inorganic salts.These findings suggest that trypsin triggered free fluoride release from Antarctic krill cuticle.In addition,the kinetics of free fluoride release could be described by the equation CW=(1-0.97-0.006t-0.03e0.0558t)×337.53+10.50.The present study improved the understanding of the role of trypsin in free fluo-ride release from Antarctic krill cuticle,facilitating future studies aimed at reducing the fluoride content in krill protein during An-tarctic krill processing.

Antarctic krillcuticlefluoridetrypsin

PENG Yuanhuai、JI Wei、JI Hongwu、HAN Zhiping、ZHANG Shiqi、WEN Yaping

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College of Food Science and Engineering,Lingnan Normal University,Zhanjiang 524048,China

College of Biological and Food Engineering,Guangdong University of Education,Guangzhou 510303,China

Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety,Guangdong Ocean University,Zhanjiang 524088,China

College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China

College of Continuing Education,Lingnan Normal University,Zhanjiang 524048,China

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Guangdong Provincial Key Laboratory of Aquatic Product Processing and SafetySchool Level Talent Project of Lingnan Normal UniversityScience and Technology Planning Project of ZhanjiangStudy on the Preparation of Active Polypeptide from the Processing Waste of White Shrimp and its Fatigue ResistanceScientific Research Capacity Improvement Project of the Key Construction Discipline of Guangdong Province

GDPKLAPPS2005ZL20210092020A010402021E050222022ZD JS079

2024

10.1007/s11802-024-5738-2

中国海洋大学学报(自然科学英文版)
中国海洋大学

中国海洋大学学报(自然科学英文版)

CSTPCD
影响因子：0.268
ISSN：1672-5182
年,卷(期)：2024.23(3)