首页|Analysis and Characterization of the GABA Transaminase and Succinate Semialdehyde Dehydrogenase Genes in the Microalga Isochrysis zhanjiangensis in Response to Abiotic Stresses

Analysis and Characterization of the GABA Transaminase and Succinate Semialdehyde Dehydrogenase Genes in the Microalga Isochrysis zhanjiangensis in Response to Abiotic Stresses

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Gamma-aminobutyric acid(GABA),widely existing in different organisms,is rapidly accumulated in plants in response to environmental stresses.The main biosynthesis and degradation pathways of GABA constitute the GABA shunt,which is tied to the tricarboxylic acid(TCA)cycle.GABA transaminase(GABA-T)and succinate semialdehyde dehydrogenase(SSADH)are two essential enzymes for the GABA degradation pathway.While there are ábundant studies on GABA shunt in higher plants at the phy-siological and genetic levels,research on its role in microalgae remains limited.This study aimed at exploring the function of GABA-T and SSADH genes in Isochrysis zhanjiangensis,an important diet microalga,under different stresses.We cloned two GABA-T ge-nes,IzGABA-T1 and IzGABA-T2,and one SSADH gene IzSSADH from Isochrysis zhanjiangensis and conducted heterologous expres-sion experiments.The results showed that the overexpression of IzGABA-T1 or IzGABA-T2 enhanced the survival rates of yeast tran-sformants under heat or NaCl stress,while the overexpression of IzSSADH improved yeast tolerance to NaCl stress but had no obvi-ous effect on heat stress.Additionally,the results of quantitative real-time polymerase chain reaction(qPCR)showed that IzGABA-T1 transcription increased in the HT(salinity 25,35℃)and LS(salinity 15,25℃)groups.At 24 h,the IzGABA-T2 transcriptions in-creased in the HT,LS,and HS(salinity 35,25℃)groups,but their transcription levels decreased in all groups at 48 h.IzSSADH tran-scription increased in the LS group.These results suggest that IzGABA-T1,IzGABA-T2,and IzSSADH are associated with tempera-ture and salinity stresses and possess a certain preference for different stresses.

abiotic stressGABAheterologous expressionIsochrysis zhanjiangensistranscription

TIAN Jiaojiao、ZHANG Lin、LU Xinyue、YE Lingzhi、WU Yuanyuan、CHEN Deshui、CAO Jiayi、JIANG Jiaxin、XU Jilin、YAN Xiaojun

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Key Laboratory of Marine Biotechnology of Zhejiang Province,School of Marine Sciences,Ningbo University,Ningbo 315211,China

Key Laboratory of Aquacultural Biotechnology Ministry of Education,Ningbo University,Ningbo 315211,China

Laizhou Bay Marine Technology Co.,Ltd.,Yantai 261400,China

Fujian Dalai Seed Science and Technology Co.,Ltd.,Ningde 352101,China

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Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Ningbo GovernmentNingbo Science and Technology Research Projects,ChinaZhejiang Provincial Department of Education Scientific Research ProjectEarmarked Fund for CARS-49K.C.Wong Magna Fund in Ningbo University

LY22C1900012021J1142019B10006Y202249030

2024

10.1007/s11802-024-5743-5

中国海洋大学学报(自然科学英文版)
中国海洋大学

中国海洋大学学报(自然科学英文版)

CSTPCD
影响因子：0.268
ISSN：1672-5182
年,卷(期)：2024.23(3)
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