首页|Characterization,Expression Pattern Analysis,and Cellular Localization of Two Homologous MUC2 Genes in Japanese Flounder(Paralichthys olivaceus)

Characterization,Expression Pattern Analysis,and Cellular Localization of Two Homologous MUC2 Genes in Japanese Flounder(Paralichthys olivaceus)

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As a member of mucins family,MUC2 is an important component of mucus,which is characterized by tandem and ir-regular repeat sequences rich in threonine and serine.It is strongly expressed by goblet cells that are involved in innate immunity.Japanese flounder is an economically important marine flatfish.In this study,two homologous genes of MUC2 were identified,named MUC2-1 and MUC2-2.Depending on phylogenetic and structural analysis,MUC2-1 and MUC2-2 were clustered in two clades,re-spectively.Paralichthys olivaceus MUC2-1 showed a closer relationship with MUC2 in the higher vertebrates.Various healthy tis-sues were analyzed to determine the expression patterns,and both MUC2 genes showed high expression levels in the gills and intes-tines.Following Edwardsiella tarda challenge,P.olivaceus MUC2-1 and MUC2-2 both showed significant up-regulated expression in intestine and kidney.Moreover,MUC2-1 was significantly up-regulated in gill cell lines following PGN and polyI:C stimulation,and MUC2-2 was significantly up-regulated in gill cell lines following LPS stimulation.In addition,ISH results revealed that MUC2-1 and MUC2-2 showed the same tissue localization in intestine tissues,but displayed different localization in gill.The siRNA-me-diated knockdown of MUC2-1 and MUC2-2 genes in the gill cell lines of Japanese flounder affected the expressions of galnt family genes and smad pathway members that are related to mucosal immunity.The results provided a valuable insight into understanding the functions of MUC2 on mucosal immune system in response to the invasion of bacterial pathogen.

MUC2ISHEdwardsiella tardaParalichthys olivaceus

ZHU Ying、YANG Xinsheng、LI Zhengjie、LI Chao

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School of Marine Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China

Natural Science Foundation of Shandong ProvinceShandong Key R&D Program for Academician Team in ShandongHigh-Level Talents Research Fund of Qingdao Agricultural UniversityYoung Experts of Taishan ScholarsShandong Technical System of Fish IndustryScience and Technology Support Plan for Youth Innovation of Colleges and Universities in Shandong Province

ZR2022QC0372023ZLYS02663/112003320 1909130SDAIT-12-032019KJF003

2024

10.1007/s11802-024-5764-0

中国海洋大学学报(自然科学英文版)
中国海洋大学

中国海洋大学学报(自然科学英文版)

CSTPCD
影响因子：0.268
ISSN：1672-5182
年,卷(期)：2024.23(4)