首页|Dietary Supplementation of β-Carotene Reveals miRNAs Involved in the Regulation of Carotenoid Metabolism in Crassostrea gigas

Dietary Supplementation of β-Carotene Reveals miRNAs Involved in the Regulation of Carotenoid Metabolism in Crassostrea gigas

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
Carotenoids play crucial physiological roles in animals.A comprehensive investigation into the mechanism of carotenoid metabolism in oysters will establish a theoretical foundation for further development of its carotenoid-rich traits.However,the in-formation on the function of miRNA in β-carotene metabolism in oysters is limited.To elucidate the mechanisms underlying miRNA regulation of carotenoid metabolism in oysters,we compared the expressions of miRNA in digestive gland tissues of Pacific oyster(Crassostrea gigas)fed with a β-carotene supplemented diet and a normal diet,respectively.A total of 690 candidate miRNAs in the Pacific oyster digestive gland tissues were identified,including 590 known miRNAs and 111 unknown miRNAs.Three differentially expressed miRNAs were obtained in the carotenoid-fed and normal groups,associated to 137 differentially expressed target genes.Moreover,the GO enrichment analysis revealed that the differentially expressed target genes were mainly involved in transmembrane transport activity.KEGG enrichment showed that the differentially expressed target genes were involved in ABC transport.Analysis of the mRNA-miRNA network revealed that novel0025 played a central role in carotenoid metabolism,and it was negatively corre-lated with the expression of 46 mRNAs.In addition,down-regulated expression of novel0025 upregulated the expression of the lipo-protein gene LOC105342186,suggesting a potential regulatory role in carotenoid metabolism.Our results provide useful information for elucidating the miRNA regulation mechanism during carotenoids metabolism in the Pacific oyster.

Crassostrea gigasβ-carotenemicroRNAcarotenoid metabolism

SUN Dongfang、WAN Sai、YU Hong、KONG Lingfeng、LI Qi

展开 >

Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China

Laboratory for Marine Fisheries Science and Food Production Processes,Laoshan Laboratory,Qingdao 266237,China

Shandong Science and Technology Small and Medium Enterprises Innovation Ability Improvement ProjectKey R&D Program of Shandong Province,ChinaChina Agriculture Research System Project

2021TSGC 12402022TZXD002CARS-49

2024

10.1007/s11802-024-5766-y

中国海洋大学学报(自然科学英文版)
中国海洋大学

中国海洋大学学报(自然科学英文版)

CSTPCD
影响因子：0.268
ISSN：1672-5182
年,卷(期)：2024.23(4)
  • 1