首页|Temperate Stutzerimonas Phage Encoding Toxin-Antitoxin System Genes Represents a Novel Genus

Temperate Stutzerimonas Phage Encoding Toxin-Antitoxin System Genes Represents a Novel Genus

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Stutzerimonas have been extensively studied due to their remarkable metabolic and physiological diversity.However,research on its phages is currently limited.In this study,we isolated a novel double-stranded DNA(dsDNA)phage,vB_SstM-PG1,from the marine environment that infects Stutzerimonas stutzeri G1.Its dsDNA genome is 37204 bp long with a G/C content of 64.14%and encodes 54 open reading frames.The phage possesses a tail packaging structure that is different from known Stutzerimonas stutzeri phages and exhibits structural protein characteristics similar to those of temperate phages.In addition,two genes of toxin-antitoxin system,including YdaS_antitoxin and HEPN_SAV_6107,were found in the vB_SstM-PG1 genome and play important roles in regu-lating host growth and metabolism.With phylogenetic tree and comparative genomic analysis,it has been determined that vB_SstM-PG1 is not closely related to any phages previously identified in the GenBank database.Instead,it has a connection with enigmatic,uncultured viruses.Specifically,the vB_SstM-PG1 virus exhibits an average nucleotide identity of over 70%with six uncultivated viruses identified in the IMG/VR v4 database.This significant finding has resulted in the identification of a novel viral genus known as Metabovirus.

StutzerimonasvB_SstM-PG1Metabovirustemperate phagegenomic and phylogenetic analysis

ZHANG Hong、GUO Ruizhe、GAO Chen、ZHENG Kaiyang、XIONG Yao、DONG Yue、LIU Yundan、WANG Ziyue、CHEN Ying、WANG Hongmin、SHAO Hongbing、SUNG Yeong Yik、MOK Wen Jye、WONG Li Lian、MCMINN Andrew、WANG Min、LIANG Yantao

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College of Marine Life Sciences,Institute of Evolution and Marine Biodiversity,MOE Laboratory of Evolution and Marine Biodiversity,Frontiers Science Center for Deep Ocean Multispheres and Earth System,Center for Ocean Carbon Neutrality,Ocean University of China,Qingdao 266003,China

UMT-OUC Joint Center for Marine Studies,Qingdao 266003,China

Institute of Marine Biotechnology,Universiti Malaysia Terengganu,21030 Kuala Terengganu,Malaysia

Institute for Marine and Antarctic Studies,University of Tasmania,Hobart,TAS 7001,Australia

Haide College,Ocean University of China,Qingdao 266100,China

The Affiliated Hospital of Qingdao University,Qingdao 266001,China

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National Natural science Foundation of ChinaNational Natural science Foundation of ChinaNational Natural science Foundation of ChinaNational Natural science Foundation of ChinaNational Natural science Foundation of ChinaFundamental Research Funds for the Central UniversitiesMarine Big Data Center of Institute for Advanced Ocean Study of Ocean University of China

42188102421201040064197611742176111423061112018 12002

2024

10.1007/s11802-024-5816-5

中国海洋大学学报(自然科学英文版)
中国海洋大学

中国海洋大学学报(自然科学英文版)

CSTPCD
影响因子：0.268
ISSN：1672-5182
年,卷(期)：2024.23(4)