首页|Effects of Different Culture Conditions and Growth States on the Structure and Hemostatic Properties of Coscinodiscus sp.
Effects of Different Culture Conditions and Growth States on the Structure and Hemostatic Properties of Coscinodiscus sp.
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As a renewable marine inorganic material,Coscinodiscus sp.has significant potential in the field of rapid hemostasis.However,the low yield of Coscinodiscus sp.seriously limits the application.In this study,two new culture modes were adopted to increase the production of Coscinodiscus sp.,the effect of changes in culture conditions and growth status on the hemostatic activity of diatoms was detected.To prevent Coscinodiscus sp.from sinking in culture,the suspension culture mode was realized by adding 0.5%agar.The semi-continuous high nutrient concentration culture mode increased the cell density of Coscinodiscus sp.to 11000 cells mL-1 and shorten the culture cycle to 5 d.In terms of coagulation activity,the addition of frustules reduced the in vitro coagula-tion time by half and the activation time of coagulation by 70%.The hemolysis rate and cytotoxicity of frustules harvested in the two culture modes did not change significantly.The results showed that suspension culture mode and high nutrient concentration culture mode only changed the growth state of Coscinodiscus sp.,while the hemostatic performance remained stable.
diatom cultureCoscinodiscus sp.frustulehemostatic materialhemorrhage control
HE Zheng、LI Qinfeng、LIU Jinfeng、HUI Guangyan、FENG Chao、CHENG Xiaojie、CHEN Xiguang
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College of Marine Life Science,Sanya Oceanographic Institution,Ocean University of China,Qingdao 266003,China
Laboratory for Marine Drugs and Bioproducts,Qingdao Marine Science and Technology Center,Qingdao 266237,China
Department of Stomatology,Qingdao Women and Children's Hospital,Qingdao 266034,China
Department of Stomatology,Qingdao Special Servicemen Recuperation Center of PLA Navy,Qingdao 266071,China
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National Natural science Foundation of ChinaSanya Science and Technology ProjectQingdao National Laboratory for Marine Science and TechnologyEducation Department of Hainan Province
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