首页|裂殖壶菌诱变筛选的研究

裂殖壶菌诱变筛选的研究

Mutation of Schizochytrium limacinum and Screening of Elite Mutants

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
首次采用紫外诱变和喹禾灵筛选的方法,选育出2株二十二碳六烯酸(Docosahexaenoic acid,DHA)含量高的裂殖壶菌(Schizoch ytrium)突变菌株OUC002和OUC007,其中OUC002生物量(6.82 g·d-1·L-1)和DHA含量(15.31％),分别比对照菌株OUC168提高8.08％和13.74％；OUC007生物量(7.04 g· d-1·L-1)和DHA含量(17.33％),比对照菌株分别提高11.57％和28.75％.通过GC-MS(气质联用)对OUC007脂肪酸组成进行分析,DHA含量达到脂肪酸的37.28％.本研究为裂殖壶菌的发酵生产提供了性状优良的菌种,并为裂殖壶菌菌种选育提供了新方法.
Schizochytrium limacinum was mutated with ultraviolet and two elite strains, OUC002 and OUC007, were screened out with quizalofop-p-ethyl. Compared with original strain, the biomass and DHA content of OUC002 were increased by 8. 08% and 13. 74%, respectively, and the biomass and DHA content of OUC007 were increased by 11. 57% and 28. 75%, respectively. As determined with GC-MS, DHA content of OUC007 reached 37. 28% of total fatty acids. These mutants promise to be used in S. limacinum fermentation. The method used in this study was effective for breeding new strains of S. limacinum.

Schizochytrium limacinumquizalofop-p-ethylultravioletfatty acid compositionDHA

许永、臧晓南、徐涤、张学成

展开 >

中国海洋大学海洋生命学院,山东青岛266003

裂殖壶菌 喹禾灵 紫外线 脂肪酸组成 DHA

国家高技术研究发展计划(863计划)国家科技支撑计划

2008AA09Z4102006BAD09A12

2012

中国海洋大学学报(自然科学版)
中国海洋大学

中国海洋大学学报(自然科学版)

CSTPCDCSCD北大核心
影响因子：0.474
ISSN：1672-5174
年,卷(期)：2012.42(12)
  • 12
  • 13