Analysis of the Composition and Changes of Epiphytic Flora in the Indoor Culture of Two Common Species of Gracilariaceae at Qingdao Coast
The growth status of macroalgae collected in the wild is often not as good as that in the wild after a period of indoor cultivation.To answer the question whether the composition and number of the epiphytic flora would change during this progress,two closed related species in Gracilariaceae,Gracilaria vermiculophylla and Gracilariopsis lemaneiformis were collected from Qingdao coast.These two species shared similar morphology and distributed nearly.The bacteria compositions and changes were analyzed and compared for these two macroalgae species during the 90 days indoor cultiva-tion.Firstly,the study evaluated the suitability of 16S rDNA specific primers(799F-1 392R)for analy-zing the epiphytic flora of Gracilaria vvermiculophylla and Gracilariopsis lemaneiformis.The primer pair 799F-1 392R was found to be effective in avoiding interference from the chloroplasts of these algae,enabling a smooth analysis of the flora structure.Next,16S rDNA sequencing was used to analyze the community diversity,community structure,and changes in the epiphytic flora of Gracilaria vermiculo-phylla and Gracilariopsis lemaneiformis.The results revealed differences between the epiphytic flora of the algae in their natural habitat and those cultured indoors.The richness of the epiphytic flora was lower in the indoor culture of Gracilariaceae compared to the wild environment.Additionally,there were differences in the composition of epiphytic flora between Gracilaria vermiculophylla and Gracilariopsis lemaneiformis,with various types of epiphytic flora showing different preferences for these two species of Gracilariaceae.The study suggested that the enormous differences between the indoor and natural en-vironments might be the main reason for the changes of epiphytic flora.Furthermore,the variation in chemical composition between the two Gracilariaceae species also influenced the bacteria attached to their surfaces.Overall,this study provides theoretical support for enhancing our understanding of the relation-ship between algae and bacteria,as well as optimizing the indoor culture of algae.
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