Genome Sequencing and Comparative Genomic Analysis of Salinicola lusitanus LLJ914
Salinicola has attracted much attention due to its strong osmotic pressure tolerance.However,the mechanism by which it adapts to high salt or other extreme marine environments is not yet known.To elucidate the differences of genetic characteristics and metabolic potential between Salinicola lusitanus LLJ914 isolated from the hydrothermal field of Okinawa Trough and bacteria in the same genus,exploring the adaptive mechanism of strain LLJ914 in extreme marine habitats,the complete genome sequence of S.lusitanus LLJ914 was obtained by whole genome sequencing.Genomes of bacteria belonging to the same genus were selected for comparative genomic analysis.The differences in metabolic potential of the genus Salinicola and S.lusitanus strains isolated from different environments were explored.The genome size of strain LLJ914 is 4 781 556 bp with a G+C content of 64.0％.It encodes a total of 4 229 proteins,69 tRNAs and 12 rRNAs.By constructing phylogenetic trees and performing average nucleotide and average amino acid consistency analyses,it was found that strain LLJ914 was most closely related to S.lusitanus CR50T from halophyte Halimione portulacoide.According to the annotation of functional genes,bacteria belonging to S.lusitanus possess various genes associated with heavy metal resistance and methanogen through methylphosphonic acid(the gene cluster phn).Compared with strain CR501,the genome of strain LLJ914 contained more genes related to amino acid and carbohydrate transport metabolism,energy production and conversion,and transcription.In addition,strain LLJ914 also harbored unique genes associated with heavy metal resistance,immune defense and aerobic respiration,which may contribute to its adaptation to the complex and extreme deep-sea hydrothermal environment.In this study,the genetic characteristics and metabolic potential of S.lusitanus LLJ914 were revealed,which should aid to understand its adaption to deep-sea hydrothermal environments.These findings provided a reference to better understanding the ecological functions of microorganisms in hydrothermal environment.
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