The Diversity of Culturable Bacteria Taiwan Cold Seep in the South China Sea
The cold seep in the South China Sea contains a large number of unique microbial resources due to its special marine environmental characteristics.This study focused on Taiwan cold seep in the South China Sea,aiming to explore the resources of uncultivable bacteria in this cold seep and reveal the diversity of cultivable bacteria.Five media including resuscitation solid medium(FSPB),resuscitation liquid enrichment medium(FSY),SOB solid medium(SOB),MMJS solid medium(MMJS)and SPM solid medium(SPM)were used to enrich and isolate microorganisms from different samples from Taiwan cold seep in the South China Sea.Through the 16S rRNA gene sequence identification,the diversity of culturable bacteria from different culture media and different samples from Taiwan cold seep in the South China Sea were analyzed and compared.In this study,690 strains were isolated and identified from seawater,sediment and biological samples from 8 stations in Taiwan cold seep in the South China Sea,which belong to six classes and 39 genera in the four phyla including Proteobacteria,Bacteroidetes,Actinobacteria and Firmicutes.Gammaproteobacteria accounted for the highest proportion of bacteria and contained 14 genera,among them Alcanivorax was the dominant.Bacteria isolated from MMJS and SPM were affiliated with the four phyla.No bacteria belonging to Actinobacteria was isolated from FSPB and FSY,and Firmicutes was not found in SOB.Bacteria from the four phyla were isolated from seawater and sediment samples,but Firmicutes were not identified in biological samples.At the class level,Gammaproteobacteria were the most abundant among three samples,and their relative abundances were higher in seawater samples than in sediment samples and biological samples.Based on a variety of samples from Taiwan cold seep in the South China Sea,our study successfully obtained numerous culturable marine bacteria by using five different media.Different media and culture methods showed both similarities and differences in the isolation of marine bacteria.Our study provided bacterial resources for further studies on their metabolic characteristics and environmental adaptation mechanism in the cold seep ecosystem.
South China Seacold seepmediumculturable bacteriumdiversity
万方数据
维普
