意大利蜜蜂乙醇胺激酶1蛋白的序列特征和同源性分析
Sequence characteristics and homology analysis of Apis mellifera ligustica Ethanolamine Kinase 1
李桂林 1夏文丽 1张呈豪 1张新怡 1赵航1
作者信息
- 1. 曲阜师范大学生命科学学院,273165,山东省曲阜市
- 折叠
摘要
运用生物信息学的方法分析意大利蜜蜂ETNK1的序列特征和同源性.理化性质分析显示,意大利蜜蜂ETNK1由348个氨基酸残基构成,相对分子量为41.54 kD,是一个带负电的稳定的亲水性蛋白.根据跨膜结构分析可知,ETNK1没有跨膜螺旋,不是跨膜蛋白.通过核定位信号和亚细胞定位分析可得,ETNK1很可能定位在细胞骨架中.蛋白质修饰位点分析表明,ETNK1有19个磷酸化位点,不含糖基化修饰位点.氨基酸多序列比对的结果显示,不同蜜蜂中的ETNK1的同源关系较高.系统进化树分析发现,意大利蜜蜂的ETNK1与蜜蜂属的大蜜蜂和黑大蜜蜂的进化关系比较相近.该研究有望为进一步研究意大利蜜蜂ETNK1的结构和功能提供支撑.
Abstract
This paper analyzes the sequence characteristics and homology of ETNK1 of Apis mellifera ligustica by using bioinformatics analysis.The analysis of physical and chemical properties shows that ET-NK1 is composed of 348 amino acid residues with a relative molecular weight of 41.54 kD,and is a nega-tively charged and stable hydrophilic protein.According to the analysis of transmembrane structure,ET-NK1 has no transmembrane helix and is not a transmembrane protein.ETNK1 is likely to be located in the cytoskeleton through the analysis of nuclear localization signal and subcellular localization.The analysis of protein modification sites shows that ETNK1 has 19 phosphorylation sites and no glycosylation sites.The results of multi-sequence alignment of amino acids show that the homology of ETNK1 in different honey-bee species is higher.Phylogenetic tree analysis shows that the ETNK1 of Apis mellifera ligustica is simi-lar to that of Apis dorsata and Apis laboriosa.This research is expected to provide support for further study on the structure and function of Apis mellifera ligustica ETNK1.
关键词
意大利蜜蜂/ETNK1/生物信息学/序列特征/同源性分析Key words
Apis mellifera ligustica/ETNK1/bioinformatics/sequence characteristics/homology analysis引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据