EXTRACTION OF IRIDOID GLYCOSIDES FROM ETHANOL-AMMONIUM SULFATE AQUEOUS TWO—PHASE SYSTEM AND VERIFICATION OF ITS ACTIVITY
Iridoid terpenes were extracted from ethanol-ammonium sulfate aqueous two-phase system with different mass fractions,and the content of iridoid terpenes was determined by using 8-O-acetylgeniposide methyl ester as standard.And its anti-inflammatory effect was verified by cell experiment.Results when the content of ethanol was 28％and the content of ammonium sulfate was 22％,the yield of iridoid glycosides could reach 327.14mg/g.And the results verified by in vitro cell anti-inflammatory experiments showed that the obtained active site of iridoid glycosides could significantly reduce the inflammation level of RAW264.7 macrophages at different doses(P＜0.05).Aqueous two-phase extraction can can effectively enrich the iridoid glycosides in Lamiophlomis rotata,and the effect is significant.
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