Stability analysis of reference gene expression in the different parts of Mandragora chinghaiensis
The roots,stems,mature fruits and mature leaves of Mandragora chinghaiensis are taken as experimental materials to explore the stable expression of reference genes in the different parts of Mandragora chinghaiensis.A total of 6 reference genes(Actin7-NT,18S rRNA,TUA,ELF4,ELF6,and GAPCP2)are selected for the study.In addition,qRT-PCR technology and five methods(geNorm,NormFinder,BestKeeper,ΔCt and RefFinder)are adopted to analyze the expression stabi-lity of candidate reference genes in order to screen the most stable reference genes in Mandragora chinghaiensis.The results show that 18S rRNA is the most stable reference gene in the roots and ma-ture leaves.ELF4 is the most stable reference gene in the stems and mature fruits.The comprehen-sive analysis results indicate that the most stable reference gene expressed in the different issues of Mandragora chinghaiensis is Actin7-NT,which provides a theoretical basis for further exploration of Mandragora chinghaiensis related genes and functional analysis of metabolism and protein.
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