青海茄参不同部位内参基因表达稳定性分析
Stability analysis of reference gene expression in the different parts of Mandragora chinghaiensis
王晓霞 1赵晓帆 1王成 1赵艳艳1
作者信息
- 1. 青海大学农牧学院,青海西宁 810016
- 折叠
摘要
为探究青海茄参不同部位稳定表达的内参基因,本研究以青海茄参根、茎、成熟果实及成熟叶片为试验材料,选取6个内参基因(Actin7-NT、18S rRNA、TUA、ELF4、ELF6和GAPCP2),采用实时荧光定量 qRT-PCR 技术,并利用 geNorm、NormFinder、BestKeeper、ΔCt 及 RefFinder 5种方法分析候选内参基因的表达稳定性,筛选青海茄参中稳定性最佳的内参基因.结果表明:18S rRNA为青海茄参根及成熟叶片中最佳稳定内参基因,ELF4为青海茄参茎及成熟果实中最稳定的内参基因.综合分析结果为青海茄参不同组织中表达最稳定的内参基因是Actin7-NT,该结果可为后续挖掘青海茄参相关基因及代谢、蛋白等功能分析提供理论依据.
Abstract
The roots,stems,mature fruits and mature leaves of Mandragora chinghaiensis are taken as experimental materials to explore the stable expression of reference genes in the different parts of Mandragora chinghaiensis.A total of 6 reference genes(Actin7-NT,18S rRNA,TUA,ELF4,ELF6,and GAPCP2)are selected for the study.In addition,qRT-PCR technology and five methods(geNorm,NormFinder,BestKeeper,ΔCt and RefFinder)are adopted to analyze the expression stabi-lity of candidate reference genes in order to screen the most stable reference genes in Mandragora chinghaiensis.The results show that 18S rRNA is the most stable reference gene in the roots and ma-ture leaves.ELF4 is the most stable reference gene in the stems and mature fruits.The comprehen-sive analysis results indicate that the most stable reference gene expressed in the different issues of Mandragora chinghaiensis is Actin7-NT,which provides a theoretical basis for further exploration of Mandragora chinghaiensis related genes and functional analysis of metabolism and protein.
关键词
青海茄参/实时荧光定量/内参基因/不同部位/稳定性Key words
Mandragora chinghaiensis/real-time fluorescence quantitative/reference genes/different parts/stability引用本文复制引用
出版年
2024
NETL
NSTL
万方数据