Construction of MSAP technology system for Lycium ruthenicum Murr.
Lycium ruthenicum Murr.DNA is extracted by adopting CTAB method in order to con-struct its methylation-sensitive amplified polymorphism(MSAP)technology reaction system utili-zing MSAP technology.Preliminary screening is conducted on the polymorphism of 16 pairs of pri mers by using double digestion,pre-amplification and selective amplification to construct MSAP technology system.The results show that DNA template can be digested completely by double diges-tion at 12 h.The bands of pre-amplification reaction products are concentrated at 250～1 000 bp.In addition,six pairs of primer combinations are screened by selective amplification with obvious bands.This study lays a foundation for the further analysis of DNA methylation level of Lycium ru-thenicum Murr.
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