Cloning and expression analysis of APRR2 gene in chili pepper with different immature fruit colors
Immature fruit color is one of the important commercial traits of Capsicum annuum L.In order to explore the effects of Arabidopsis pseudo response regulator gene(APRR2)on the imma-ture fruit color,Qinghai local variety Xunhua chili pepper dark green immature fruit color wild type XHB and light green immature fruit color mutant QL2017 are taken as research materials to clone and analyze the spatial and temporal expression of APRR2 gene with different immature fruit colors of Capsicum annuum L.The results show that the full-length of CDS sequence of APRR2 gene is 1 737 bp,encoding 578 amino acids.There is no difference between XHB and QL2017 sequences.The APRR2 protein has a molecular weight of 64.34 kDa and an isoelectric point of 5.74.It is an unstable hydrophilic protein,which belongs to SANT superfamily.In terms of systematic evolutionary relationship,APRR2 protein of chili pepper belongs to the same branch as Solanaceae tomato and Panari tomato.The expressions of APRR2 gene are significantly different in different tissue of the two varieties at different stages with tissue specificity.The expression levels of APRR2 gene in XHB fruit,QL2017 leaf and fruit are significantly higher than that in other tissue,while the expression level in stem is the lowest.The results can provide a theoretical basis for subsequent related gene mapping and in-depth analysis of formation mechanism of immature fruit colors of chili pepper.
Capsicum annuum L.immature fruit colorAPRR2 genegene cloninggene expression
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万方数据
