The exploration of the characteristics and biological functions of the AsSWEETs gene family in Allium sativum L.is a way to provide reference for the molecular mechanism of its bulb develop-ment regulation.Based on the whole genome data of Allium sativum L."Ershuizao",members of As-SWEETs are screened and identified through HMM search.Their expression patterns under different hormones are analyzed by adopting real-time fluorescence quantitative PCR.The results indicate that a total of 35 AsSWEETs genes are identified in the Allium sativum L.genome,all of which are hydrophobic proteins with 2～7 different transmembrane helices.Except for AsSWEET1 and As-SWEET2 located in scaffold,the rest are distributed on chromosomes with significant differences among members.Through comprehensive systematic evolution and expression patterns,it is found that AsSWEET5,14,21,23,26,28(Ⅲ branch,transporting sucrose)may be the main regulatory gene involved in the bulb enlargement.The expression levels of AsSWEET2,30 and 33(Ⅳ branch)vary greatly at different stages of bulb development,which may be involved in the regulation of fructose metabolism during the bulb development.According to qRT-PCR quantitative analysis,AsSWEET5,11 and 16 show different expression patterns under different hormone treatments,indicating that there may be differentiation and differences in the functions of AsSWEETs family members.In summary,the sugar transport activity of Allium sativum L.AsSWEETs gene is involved in the growth and develop-ment of bulb,which helps explain the process of bulb enlargement and development.
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