In order to solve the problem of low removal efficiency of ammonia nitrogen and nitrate in the treatment of aquaculture waste water by traditional aeration biological filters,the effects of diffe-rent carbon sources and voltage intensities on the removal efficiency of chemical oxygen demand(COD),ammonia nitrogen(NH3-N),nitrate nitrogen(NO3--N),total nitrogen(TN)and total phosphorus(TP)in microbial electrolysis cell(MEC)system are investigated by using MEC me thod at low temperatures.The aim is to explore how MEC enhance water treatment capacity of fresh-water cold-water fish culture system under the conditions of optimal carbon source and voltage in-tensity,and to explore its removal mechanism.The results show that when sodium acetate is carbon source,the removal rates of COD,NH3-N,NO3--N and TP reach 82.10%,87.56%,98.64%and 73.64%respectively.When the voltage intensity is 0.30 V,the removal rates of COD,NH3-N,NO3--N and TP are 81.01%,73.15%,76.74%and 83.97%respectively.Marco gene sequen-cing results show that under the optimal carbon source and voltage intensity,Streptomyces,Pseudo-monas and Burkholderia are the dominant genera in the microbial community at genus level.The key functional genes amoCAB,nxrAB,nirBD,nrfA and nifDHK involved in the nitrogen metabolism path-way show that MEC system mainly undergoes nitrogen removal metabolism through the pathway of simultaneous nitrification and denitrification.
cold-water fish farmingmicrobial electrolysis cell(MEC)carbon sourceapplied voltagemicrobial community