Virus disease is one of the important factors affecting the yield and seed quality of Sola-num tuberosum L..In order to explore the main virus species of Solanum tuberosum L.in Qinghai re-gion,245 samples of the suspected diseased plant leaves were collected from the production fields of Huangzhong District,Datong Hui and Tu Autonomous County,Huangyuan County,Huzhu Tu Autono-mous County and Ledu District in Qinghai from 2019 to 2020.A total of 201 samples of seed were collected from different grades of Solanum tuberosum L..Enzyme linked immunosorbent assay(DAS-ELISA)is used to screen seven major viruses in Solanum tuberosum L.,including PVY,PVX,PL-RV,PVA,PVM,PVS and AM V.The results show that the virus is detected in 105 samples,and the detection rate of PVY virus is the highest(28.98％),following is PVS,with a detection rate of 21.22％.The detection rates of PLRV,PVX,PVM and PVA are relatively low,at 5.31％,4.90％,4.49％and 2.86％respectively,while AMV is not detected.The co-infection of PVY+PVS is common,with an infection rate of 10.20％.No co-infection of three or more viruses is found.There are significant differences in virus species and virus carrying rate in Solanum tuberosum L.fields in different counties.The virus detection results of different grades of Solanum tuberosum L.are in line with its quality standard.
Solanum tuberosum L.virus diseaseinvestigation and identificationDAS-ELISA
NETL
NSTL
万方数据
