Identification of resistance to late blight in Solanum tuberosum L.progeny materials
In order to screen out progeny materials with excellent agronomic properties and disease resistance,four types of strains are inoculated on the in vitro leaves of 60 pieces of hybrid progeny materials of Qingshu No.9 and Daxiyang of Solanum tuberosum L.The disease spot area and resis tance grade of the 4 strains are analyzed after inoculation.The technology loop-mediated isothermal amplification(LAMP)is adopted to amplify in vitro leaves inoculated on different days.The results show that among the 60 pieces,6 pieces are highly resistant,11 are resistant,17 are moderately re-sistant,24 are susceptible and 2 are highly susceptible.There are a total of 34 pieces of progeny ma-terials exhibiting disease resistance,all of which have broad-spectrum characteristics.Among them,18 pieces of progeny materials all show disease resistance to the 4 strains,and 16 pieces show disease resistance to 3 or 2 strains.The broad-spectrum of progeny materials that show resistance to the 4 strains is higher than that of the materials showing resistance to 3 or 2 strains.LAMP is adopted to detect late blight pathogen in the in vitro leaves within 1～5 days of inoculation and 5 days after inoculation.The amplification test results are consistent,all blue,indicating positivity.In conclusion,the progeny materials of Solanum tuberosum L.screened in the study can provide variety reference for disease resistance breeding.LAMP can achieve the purpose of early identification and warning of So-lanum tuberosum L.late blight,thereby providing technical support for early control of the diease.
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