摘要
为了解西宁市越冬夜栖乌鸦群体携带病毒的本底情况,以市区冬季夜栖秃鼻乌鸦、大嘴乌鸦和小嘴乌鸦的新鲜粪便为试验材料,利用宏病毒组学测序技术分析乌鸦粪便病毒的组成、丰度和基因功能.结果表明:共鉴定出7 411条DNA病毒contigs和224条RNA病毒contigs.在数量前10的DNA病毒contigs中注释到9个病毒科,在数量前5的RNA病毒contigs中注释到4个病毒科,涵盖了植物、脊椎动物、节肢动物和原生动物等来源的病毒及噬菌体.从DNA病毒contigs和RNA病毒contigs中分别预测出9 226个基因和111个基因.基因数量最多的前5个功能类别分别是遗传信息处理、复制和修复、信号传导和细胞过程、核苷酸代谢和氨基酸代谢.研究结果可为深入探究病毒进化及跨物种传播规律提供数据参考.
Abstract
The fresh feces of Corvus frugilegus,Corvus macrorhynchos and Corvus corone in the urban area of Xining is taken as the experimental materials to investigate the background situation of the vi-rus carried by the overwintering nocturnal Corvus sp.population in Xining.Virome omics sequencing technology is adopted to reveal the composition,abundance and genetic function of fecal virus of Cor-vus sp.The results show that a total of 7 411 DNA virus contigs and 224 RNA virus contigs are identified.The top 10 DNA virus contigs are annotated into 9 viral families,while the top 5 RNA vi-rus contigs are annotated into 4 viral families,covering viruses and phages from plant,vertebrate,ar-thropod and protozoa orgins.A total of 9 226 genes and 111 genes are predicted for DNA virus contigs and RNA virus contigs respectively.The top 5 functional categories with the highest number of genes are genetic information processing,replication and repair,signal conduction and cellular process,nucleotide metabolism and amino acid metabolism respectively.The research results can provide basic data reference for in-depth exploration of virus evolution and cross species transmission patterns.
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