Enzymolysis technology and property research of Scomberomorus niphonius bones antioxidant peptide
To further investigate the enzymolysis technology and properties of Scomberomorus nipho-nius bones antioxidant peptide,so as to promote the development and utilization of fish bone,Scom-beromorus niphonius bones are taken as raw materials to evaluate its in-vitro antioxidant capacity using DPPH,ABTS and FRAP methods,and to determine enzymolysis technology using response sur-face optimization experiments.The possible antioxidant mechanism of Scomberomorus niphonius bones polypeptide is explored by analyzing the molecular weight and amino acid composition of polypep-tide.The results are as follows:(1)The antioxidant effect of polypeptide hydrolyzed by alkaline pro-tease is better,and the optimal conditions are achieved at a solid-liquid ratio of 1:30,enzyme addi-tion of 14 000 U/g,enzymatic hydrolysis pH 6.4,enzymatic hydrolysis time of 2.0 h and enzymatic hydrolysis temperature of 52 ℃.(2)The DPPH radical scavenging rate of Scomberomorus niphonius bones antioxidant peptide is 63.47％,ABTS radical scavenging rate is 73.89％,and iron ion reducing ability is 1 099.27 μmol TE/g DW;97.07％of the molecular weight is below 3 000 Da,and 82.08％is below 1 000 Da.(3)Scomberomorus niphonius bones antioxidant peptide is rich in 17 amino acids such as glycine and glutamate,with a total content of 49.07％.The research results can provide data reference for the study of Scomberomorus niphonius bones antioxidant peptide and the development of foodborne antioxidant peptide products.
Scomberomorus niphonius bonesantioxidant peptideenzymolysis technologyproperty research
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