马鲛鱼骨抗氧化肽的酶解工艺及性质研究
Enzymolysis technology and property research of Scomberomorus niphonius bones antioxidant peptide
洪燕婷 1颜阿娜 1汪少芸 2蔡茜茜2
作者信息
- 1. 黎明职业大学轻工学院,福建泉州 362000
- 2. 福州大学生物科学与工程学院,福建 福州 350108
- 折叠
摘要
为进一步探究马鲛鱼骨抗氧化肽的酶解工艺及其性质,促进对鱼骨的开发利用,本文以马鲛鱼骨为原料,采用DPPH法、ABTS法和FRAP法评价其体外抗氧化能力,并通过响应面优化实验确定酶解工艺,通过分析多肽的分子量和氨基酸组成,探索马鲛鱼骨多肽可能的抗氧化机理.结果表明:(1)碱性蛋白酶解的多肽抗氧化效果较佳,在料液比1∶30,酶添加量14 000 U/g,酶解pH 6.4,酶解时间2.0 h,酶解温度52℃时达到最优.(2)马鲛鱼骨抗氧化肽的DPPH自由基清除率为63.47％,ABTS自由基清除率为73.89％,铁离子还原能力为1 099.27 μmol TE/g DW;97.07％的分子量在3 000 Da以下,82.08％的分子量在1 000 Da以下.(3)马鲛鱼骨抗氧化肽富含甘氨酸、谷氨酸等17种氨基酸,总含量达49.07％.研究结果可为马鲛鱼骨抗氧化肽相关研究及食源性抗氧化肽产品的开发提供数据参考.
Abstract
To further investigate the enzymolysis technology and properties of Scomberomorus nipho-nius bones antioxidant peptide,so as to promote the development and utilization of fish bone,Scom-beromorus niphonius bones are taken as raw materials to evaluate its in-vitro antioxidant capacity using DPPH,ABTS and FRAP methods,and to determine enzymolysis technology using response sur-face optimization experiments.The possible antioxidant mechanism of Scomberomorus niphonius bones polypeptide is explored by analyzing the molecular weight and amino acid composition of polypep-tide.The results are as follows:(1)The antioxidant effect of polypeptide hydrolyzed by alkaline pro-tease is better,and the optimal conditions are achieved at a solid-liquid ratio of 1:30,enzyme addi-tion of 14 000 U/g,enzymatic hydrolysis pH 6.4,enzymatic hydrolysis time of 2.0 h and enzymatic hydrolysis temperature of 52 ℃.(2)The DPPH radical scavenging rate of Scomberomorus niphonius bones antioxidant peptide is 63.47％,ABTS radical scavenging rate is 73.89％,and iron ion reducing ability is 1 099.27 μmol TE/g DW;97.07％of the molecular weight is below 3 000 Da,and 82.08％is below 1 000 Da.(3)Scomberomorus niphonius bones antioxidant peptide is rich in 17 amino acids such as glycine and glutamate,with a total content of 49.07％.The research results can provide data reference for the study of Scomberomorus niphonius bones antioxidant peptide and the development of foodborne antioxidant peptide products.
关键词
马鲛鱼骨/抗氧化肽/酶解工艺/性质研究Key words
Scomberomorus niphonius bones/antioxidant peptide/enzymolysis technology/property research引用本文复制引用
出版年
2024
NETL
NSTL
万方数据