Effects of Sodium Selenite Treatment on Physiological Indexes Related to Oilseed Rape Seedlings
In this paper,the effects of different concentrations of sodium selenite(Na2SeO3)on the growth,development and physiological characteristics of oilseed rape seedlings were investigated,the mechanism of physiological response of oilseed rape seedlings to selenium stress was revealed,to provide a theoretical basis for the breeding of selenium-enriched oilseed rape and the production practice.Seedlings were cultivated in pots for 1 week,and then treated with Na2SeO3 at concentrations of 0.000,0.010,0.020,0.050,0.075,0.100 g/kg for 2 weeks,and then relevant morphological and physiological indexes were measured in the seedlings.Compared with the control,the changes in growth and physiological indexes of oilseed rape seedlings with the increase of Na2SeO3 concentration included:(1)The increase of plant height,leaf length,dry weight and fresh weight of oilseed rape seedlings with Na2SeO3 treatment concentration of 0.020 g/kg promoted the growth of seedlings,while the treatment concentration of 0.100 g/kg inhibited the growth of seedlings.(2)When the concentration of Na2SeO3 was 0.075 g/kg,the contents of chlorophyll a,chlorophyll b,chlorophyll a+b and carotenoids increased significantly by 36.85%,69.41%,53.14%and 156.08%,respectively(P<0.05).(3)When the concentration of Na2SeO3 was 0.020 g/kg,the contents of soluble sugar,proline and hydrogen peroxide in rapeseed seedlings increased significantly by 47.38%,377.21%and 135.93%,respectively(P<0.05).The MDA content showed a decreasing trend,and the malondialdehyde(MDA)content decreased significantly by 25.47%and 26.24%at the treatment concentrations of 0.075 g/kg and 0.100 g/kg(P<0.05).(4)When the concentration of Na2SeO3 was 0.020-0.075 g/kg,the ascorbate peroxidase(APX)activity increased significantly by 35.10%-72.02%(P<0.05),and decreased by 99.71%when the treatment concentration was 0.100 g/kg(P<0.05).When the concentration of Na2SeO3 was 0.020-0.100 g/kg,the peroxidase(POD)activity increased significantly by 23.24%-78.42%(P<0.05).The activity of superoxide dismutase(SOD)decreased significantly by 22.39%-31.22%(P<0.05).(5)The concentration of Na2SeO3 was significantly positively correlated with carotenoid content and O2.-production rate(P<0.01),and negatively correlated with dry weight,fresh weight,MDA content and SOD activity of seedlings(P<0.01).Therefore,different Na2SeO3 concentrations had different effects on the growth and physiological characteristics of rapeseed seedlings,and the best effect was achieved at 0.020 g/kg Na2SeO3 concentration.
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