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低温胁迫下6种小檗属植物生理特性及耐寒性评价

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以青海省6种小檗属植物的休眠枝条为试材,采用人工梯度降温的方法,分别在-20℃、-25℃、-30℃、-35℃、-40℃下处理 24 h,以 4℃为对照。研究了低温处理后各小檗枝条各项生理指标。结果表明:随着处理温度的降低,拟小檗的相对电导率呈持续下降趋势,鲜黄小檗的相对电导率呈持续上升趋势,刺檗、直穗小檗、匙叶小檗、细叶小檗的相对电导率呈先上升后下降趋势;拟小檗、匙叶小檗PRO含量呈持续上升趋势,刺檗、鲜黄小檗、直穗小檗、细叶小檗PRO 含量呈持续下降趋势;MDA、POD、SOD、SS、SP变化一致,呈先上升后下降趋势。通过计算各单项指标的抗寒系数,建立隶属函数进行评价,评价结果为鲜黄小檗﹥直穗小檗﹥刺檗﹥拟小檗﹥匙叶小檗﹥细叶小檗。
Evaluation of the Physiological Characteristics and Cold Tolerance of Six Berberis Under Low Temperature Stress
The dormant branches of six Berberis plants in Qinghai Province were used as test materials,and the artificial gradient cooling method was used to treat them at-20 ℃,-25 ℃,-30 ℃,-35 ℃,-40 ℃ for 24 h,with 4 ℃ as the control.The physiological indexes of Berberis branches after low temperature treatment were studied.The results showed that with the decrease of treatment temperature,the relative conductivity of Berberis dubia showed a continuous downward trend,the relative conductivity of Berberis diaphana showed a continuous upward trend,and the relative conductivity of Berberis vulgaris,Berberis dasystachya,Berberis vernae and Berberis poiretii showed a trend of increasing first and then decreasing.The PRO content of Berberis dubia and Berberis vernae showed a continuous upward trend,while the PRO content of Berberis vulgaris,Berberis diaphana,Berberis dasystachya and Berberis poiretii showed a continuous downward trend.The changes of MDA,POD,SOD,SS and SP were consistent,showing a trend of increasing first and then decreasing.By calculating the cold resistance coefficient of each single index,the membership function is established for evaluation.The evaluation results were as follows:Berberis diaphana>Berberis dasystachya>Berberis vulgaris>Berberis dubia>Berberis vernae>Berberis poiretii.

BerberisArtificial low temperature stressMembership function method

田新格、何振华、顾文毅、祁银燕

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青海大学农林科学院/青海省农林科学院青海省高原林木遗传育种重点实验室,青海高原林木遗传育种国家林业和草原局重点实验室,西宁 810016

循化撒拉族自治县自然资源和林业草原局,海东 811100

青藏高原种质资源研究与利用实验室,西宁 810016

小檗 人工低温胁迫 隶属函数法

2024

青海科技
青海省科学技术厅

青海科技

影响因子:0.052
ISSN:1005-9393
年,卷(期):2024.31(2)