摘要
以青海省6种小檗属植物的休眠枝条为试材,采用人工梯度降温的方法,分别在-20℃、-25℃、-30℃、-35℃、-40℃下处理 24 h,以 4℃为对照.研究了低温处理后各小檗枝条各项生理指标.结果表明:随着处理温度的降低,拟小檗的相对电导率呈持续下降趋势,鲜黄小檗的相对电导率呈持续上升趋势,刺檗、直穗小檗、匙叶小檗、细叶小檗的相对电导率呈先上升后下降趋势;拟小檗、匙叶小檗PRO含量呈持续上升趋势,刺檗、鲜黄小檗、直穗小檗、细叶小檗PRO 含量呈持续下降趋势;MDA、POD、SOD、SS、SP变化一致,呈先上升后下降趋势.通过计算各单项指标的抗寒系数,建立隶属函数进行评价,评价结果为鲜黄小檗﹥直穗小檗﹥刺檗﹥拟小檗﹥匙叶小檗﹥细叶小檗.
Abstract
The dormant branches of six Berberis plants in Qinghai Province were used as test materials,and the artificial gradient cooling method was used to treat them at-20 ℃,-25 ℃,-30 ℃,-35 ℃,-40 ℃ for 24 h,with 4 ℃ as the control.The physiological indexes of Berberis branches after low temperature treatment were studied.The results showed that with the decrease of treatment temperature,the relative conductivity of Berberis dubia showed a continuous downward trend,the relative conductivity of Berberis diaphana showed a continuous upward trend,and the relative conductivity of Berberis vulgaris,Berberis dasystachya,Berberis vernae and Berberis poiretii showed a trend of increasing first and then decreasing.The PRO content of Berberis dubia and Berberis vernae showed a continuous upward trend,while the PRO content of Berberis vulgaris,Berberis diaphana,Berberis dasystachya and Berberis poiretii showed a continuous downward trend.The changes of MDA,POD,SOD,SS and SP were consistent,showing a trend of increasing first and then decreasing.By calculating the cold resistance coefficient of each single index,the membership function is established for evaluation.The evaluation results were as follows:Berberis diaphana>Berberis dasystachya>Berberis vulgaris>Berberis dubia>Berberis vernae>Berberis poiretii.
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万方数据