Optimization of the Extraction Process of Volatile Oil of Nardostachys jatamansi and Comparative Analysis of Its Chemical Constituents by GC-MS and SPME-GC-MS Methods
Optimization of the Extraction Process of Volatile Oil of Nardostachys jatamansi and Comparative Analysis of Its Chemical Constituents by GC-MS and SPME-GC-MS Methods
Objective:To optimize the extraction process of volatile oil from Nardostachys jatamansi and to analyze its volatile components.Methods:The effects of salt addition,material-liquid ratio,soaking time,and extraction time on the content of N.jatamansi volatile oil were investigated through single-factor experiments.The Box-Behnken response surface method was employed to establish a mathematical regression model,using the content of N.jatamansi volatile oil as the evaluation index.Gas chromatography-mass spectrometry(GC-MS)and solid-phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS)were utilized to analyze the components of the volatile oil.Results:Based on the optimization results from the Box-Behnken response surface design and the fitted regression equation,the optimal extraction parameters were determined to be an extraction time of 10.158 hours,a material-liquid ratio of 1:26.476,a salt addition of 3.016％,and a grinding granularity of 43.306 mesh.Under these conditions,the yield of N.jatamansi volatile oil was 4.278％.A total of 62 components were identified by GC-MS analysis,and 38 components were identified by SPME-GC-MS analysis.Conclusion:Under these extraction experimental conditions,the extraction rate of N.jatamansi volatile oil was stable,and the GC-MS analysis conditions allowed for the analysis of a more desirable range of volatile oil components.
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