Objective:To establish the quality standard for Tibetan Zha'erxiu Ointment.Methods:Thin-layer chromatography(TLC)was used for the qualitative identification of ingredients,including Curcuma longa and Glycyrrhiza uralensis.High-performance liquid chromatography(HPLC)was employed to determine the content of curcumin in the preparation.The chromatographic column was an Ultimate XB-C18(4.6 mm × 250 mm,5 μ m),with a mobile phase of acetonitrile-4％glacial acetic acid solution(48∶52),a flow rate of 1.0 mL/min,a column temperature of 30℃,a detection wavelength of 430nm,and an injection volume of 5 μ L.Results:The TLC identification spots for Curcuma longa and Glycyrrhiza uralensis were clear,with good separation and no negative interference.The linear range of curcumin was 0.694 to 69.386μg/mL(R=0.999,9).The relative standard deviations(RSDs)for precision,stability,and repeatability tests were all less than 3.0％.The mean recovery rate of curcumin was 98.55％,with an RSD of 1.1％(n=9).The curcumin content in three batches of samples was 1.073 mg/g,1.069 mg/g,and 1.072 mg/g,respectively.Conclusion:The established method is accurate and reliable,suitable for quality control of Tibetan Zha'erxiu Ointment.
维普
万方数据