To isolate and identify the pathogens resulting Citrus maxima anthracnose as well as study its pathogenicity and biological characteristics,tissues isolation was used to isolate and purify the pathogens of an-thracnose in C.maxima.In vitro leaf inoculation test was used to confirm the pathogenic of the isolated poten-tial pathogens,and its taxonomic status was determined by morphological observation,sequence homology com-parison and phylogenetic analysis of ITS gene.Biological characteristics of the fungal was determined by single factor test.The results indicated that the isolated C.maxima pathogenic fungi named YZ4 was identified as Colletotrichum gloeosporioides.The most suitable carbon and nitrogen sources for YZ4 growth were soluble starch and peptone,respectively.The optimal growth pH of the strain was 5.0.The above results provide basic data for the management of anthracnose in C.maxima.
维普
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