摘要
本试验以保存不同年份的野生大豆种子作为试验材料,研究水杨酸(SA)对野生大豆种子萌发和幼苗生理特性的影响.结果显示:水杨酸能够提高不同保存时间的野生大豆的萌发指标.2006年样本发芽势、2011年常温保存样本发芽率、2011年冷藏的样本发芽指数,与相应的对照比分别升高了 29.77%、47%、4.59%.从三种抗氧化酶活性结果来看,2008年样本的SOD活性、POD活性、CAT活性的增长率均最高,分别为-4.14%、-7.79%、-17.12%.2006年样本的可溶性糖含量的增长率最高,为59.90%.水杨酸可以增加野生大豆可溶性糖含量,降低了 POD、SOD、CAT等保护酶的酶活性,从而促进了种子的萌发.水杨酸在调控野生大豆种子衰老过程中发挥着积极的作用.
Abstract
Wild soybean seeds from different years were taken as the object,the effects of salicylic acid(SA)on the germination and physiological characteristics of wild soybean seeds were studied.The results showed that SA could improve the germination indicators of wild soybeans with different storage times.The ger-mination potential of samples in 2006,germination rate of samples stored at room temperature in 2011,and germination index of samples refrigerated in 2011 increased by 29.77%,47%,and 4.59%compared to the corresponding control group,respectively.From the results of three antioxidant enzyme activities,the growth rates of SOD activity,POD activity,and CAT activity in the samples in 2008 were the highest,with values of-4.14%,-7.79%,and-17.12%,respectively.The growth rate of soluble sugar content in the samples was the highest in 2006,at 59.90%.SA can increase the soluble sugar content of wild soybeans,reduce the enzyme activity of protective enzymes such as POD,SOD,CAT,and promote seed germination.SA plays a pos-itive role in regulating the aging process of wild soybean seeds.
基金项目
国家自然科学基金(21701021)
德州市科技计划(2022dzkj040)
大学生科技创新创业训练计划(2023)(X202310448029)
德州学院自然科学横向课题(HXKT2022190)