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麻花艽内生真菌群落多样性及土壤理化因子相关性研究

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本文以高寒草甸的麻花艽(Gentiana straminea Maxim.)为研究对象,采用组织块培养法对麻花艽根、花、茎和叶四个组织部位的内生真菌进行分离,经形态学及ITS测序鉴定后分析麻花艽内生真菌的多样性.结果显示,麻花艽不同组织部位中共获得268株内生真菌,共聚类为40个OTUs.根、花、茎和叶部位的较优势菌属依次为刚毛藻属(Cladophora),灰葡萄孢属(Botrytis),枝孢菌属(Cladosporium)和壳针孢菌属(Septoria).内生真菌群落多样性指数(Shannon指数、Simpson指数)均为根部较高.根和茎部位内生真菌群落结构相近,差异较小.土壤pH、全氮、全钾、速效磷和容重是麻花艽内生真菌群落多样性的主要影响因子.
Correlation between the Diversity of Endophytic Fungal Communities in Gentiana Straminea Maxim.and Soil Physicochemical Factors
The endophytic fungi of Gentiana straminea Maxim.from alpine meadows were isolated from four tissue parts:roots,flowers,stems and leaves by using tissue block culture method.The diversity of endo-phytic fungi of G.straminea was analysed after morphological and ITS sequencing identification.As the re-sults showed,a total of 268 endophytic fungal strains were obtained from different tissue parts of G.stra-minea,which were classified into 40 OTUs.In the order of more dominant genera in the root,flower,stem and leaf parts were Cladophora,Botrytis,Cladosporium and Septoria.The endophytic fungal community diver-sity indices(Shannon's index,Simpson's index)were all higher in the roots.The endophytic fungi community structure in the root and stem parts was similar and less different.Soil pH,total nitrogen,total potassium,quick-acting phosphorus and bulk weight were the main factors influencing the diversity of endophytic fungal communities of G.straminea.

Gentiana straminea Maxim.Endophytic fungiα diversitySoil physicochemical properties

郑坤、胡星强、孟静、叶星、王敏、周党卫

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青海民族大学药学院青藏高原植物化学重点实验室,青海 西宁 810007

中国科学院西北高原生物研究所高原生物适应与进化重点实验室,青海 西宁 810008

麻花艽 内生真菌 α多样性 土壤理化性质

2024

青海农林科技
青海省农林科学院 青海省林学会 青海省农学会

青海农林科技

影响因子:0.183
ISSN:1004-9967
年,卷(期):2024.(3)