Effects of UV-B Radiation on the Synthesis of Flavonoids and Carotenoids in Lycium barbarum
In order to understand the changes in the synthesis and accumulation of active components in Lycium barbarum fruit under different light conditions,and to study the effects on flavonoids and carotenoids synthesis and the regulatory mechanisms,Lycium barbarum fruit was subjected to ultraviolet(UV)radiation and analyzed by transcriptome sequencing.It was found that moderate UV radiation significantly increased the content of flavonoids and carotenoids in Lycium barbarum fruits,in which the expression of flavonoid synthesis-related genes CHS,HCT,F3H and FLS,as well as the carotenoid synthesis-related genes PSY and CYP707A,was significantly up-regulated.The analysis of the GO and KEGG pathways indicated that these genes were mainly involved in cellular metabolism and signaling processes,and promoted the synthesis of raw pinein chalcone,dihydrokaempferol,dendrobium flavonoids,populinone and lycopene.This study provides a scientific basis for the use of UV radiation to regulate the synthesis of active ingredients in Lycium barbarum.