Taking the stem segments with buds germinated in spring of Syringa persica as explants,by setting the basic medium types and different concentrations of cytokinin(6BA),indolebutyric acid(IBA)and a-naphthylacetic acid(NAA),the screening experiments of the best induction medium,bud prolifera-tion medium and aseptic seedling rooting medium were carried out.The results showed that the best induction medium was MS+6BA 2.0 mg/L;The optimum medium for bud proliferation was MS+6BA 3.0 mg/L+IBA 0.5 mg/L;The best rooting medium for aseptic seedlings was 1/2 MS+IBA 0.5 mg/L+NAA 0.1 mg/L.