Determine and Analysis of Leaf Transcriptome of Nitraria tangutorum
In order to understand the genetic characteristics of Nitraria tangutorum Bobr.at the genetic level,this paper took the leaves of Nitraria tangutorum Bobr.as materials for transcriptome analysis using high-throughput sequencing technology.The gene annotation results showed that a total of 28 614 Unigenes were annotated,and 3 176 were jointly annotated by seven major databases.The NR annotation results indica-ted that the gene sequence of Nitraria tangutorum Bobr.leaves had the highest homology sequence matching degree with sweet oranges.The gene annotation in the GO database was mainly concentrated in cellular processes,metabolic processes,and binding and catalytic activities.In the KEGG database,a total of 316 genes were enriched in the cellular process branch and 250 genes were enriched in the environmental informa-tion processing branch.Among them,the plant hormone signal-regulated metabolic pathway played an im-portant role in the drought regulation process.The branch with the most genes annotated was 223.In the me-tabolism branch,significantly expressed genes were more frequently annotated in metabolic pathways such as amino acid metabolism pathways and phenylpropanoid biosynthesis,which were related to the stress tolerance of Nitraria tangutorum Bobr.and the harsh environment of its habitat.