In order to identify the leaf spot pathogen of oat and filtrate the control agents of the pathogen,we i-dentified the pathogen of oat leaf spot in Dalong Village,Datong County,Xining City,Qinghai Province based on morphological characteristics and rDNA-ITS sequence analysis.Then,the effects of different carbon and nitrogen sources on mycelia growth of pathogens were determined.Furthermore,the inhibition effects of 8 fungicides on the fungi pathogens were also determined using the mycelial growth rate method.The results showed that 43 isolates from leaf spot were all the Drechslera avenae.Comparing the effects of 7 carbon sources and 8 nitrogen sources on mycelia growth found that fructose and yeast extract had the best acceleration.Laboratory filtrating test demonstra-ted that pyrazolesterin and fludioxonil both had a good inhibitory effect on D.avenaeand the EC50 values were 0.894 mg·L-1 and 3.656 mg·L-1,respectively.However,the inhibition effects of difenoconazole,tebuconazole and metiram were poor.Concretely,the EC50values ranged from 9.145 to 3.656 mg·L-1.The results would pro-vide a scientific basis for controlling oat leaf spot in the process of production.
万方数据
维普
