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三种疫苗对牦牛免疫血清抗体效价监测分析

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为监测牛病毒性/黏膜病灭活疫苗、牛副伤寒灭活疫苗及多杀性巴氏杆菌病灭活疫苗对牦牛的免疫效果,本试验选取健康牦牛40 头,以多杀性巴氏杆菌病灭活疫苗每头 4mL的剂量、牛病毒性/黏膜病灭活疫苗及牛副伤寒灭活疫苗每头2mL的剂量进行接种,并于免疫后30、60、90、120、150 和180 d后采集血液,采用酶联免疫吸附测定(ELISA)法对牦牛血清抗体进行检测.结果表明,病毒性/黏膜病灭活疫苗免疫保护效果较好,免疫 6 个月后能维持较高的血清抗体效价;牛副伤寒灭活疫苗及多杀性巴氏杆菌灭活疫苗免疫抗体效价较低.本试验对免疫后抗体的监测评估为今后牦牛三种疫病的疫苗免疫防控提供了数据支撑.
Monitoring and Analysis of Serum Antibody Potency of Three Vaccines Against Yak
In order to monitor the immunization effect of inactivated bovine viral/mucosal disease vaccine,in-activated bovine paratyphoid fever vaccine and inactivated multicidal bartonellosis vaccine on yaks,40 healthy yaks were selected in the present research and vaccinated with inactivated multicidal bartonellosis vaccine at a dose of 4 mL/head、inactivated bovine viral/mucosal disease vaccine and inactivated bovine paratyphoid fever vaccine at a dose of 2 mL/head.The serum antibodies of the yaks were detected using enzyme-linked immunosorbent assay(ELISA)by collecting the blood at 30,60,90,120,150,and 180 d after immunization.The results showed that the inactivated viral/mucosal disease vaccine had a better immunoprotective effect and maintained a higher serum antibody potency after 6 months of immunization;the inactivated bovine paratyphoid vaccine and the inactivated Pasteurella multocida vaccine had a lower immunological antibody potency.The monitoring and evaluation of post-immunization antibodies in this research provides data support for future vaccine immunization and control of the three epidemics in yak.

YakBbovine viral diarrheaSalmonella bovis paratyphiPasteurella multocidaInactivated vac-cine

章石楠、李生庆、石田、李淑萍、高瑞、胡国元、韩生义

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青海大学畜牧兽医科学院,青海省动物疫病病原诊断与绿色防控技术研究重点实验室,青海 西宁 810016

牦牛 牛病毒性腹泻 牛副伤寒沙门氏菌 多杀性巴氏杆菌 灭活疫苗

青海省科学技术厅科技成果转化专项项目

2022-NK-118

2024

青海畜牧兽医杂志
青海省畜牧兽医学会 青海省畜牧兽医科学院

青海畜牧兽医杂志

影响因子:0.196
ISSN:1003-7950
年,卷(期):2024.54(1)
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