Quality Analysis of Thawing Fine Tube Frozen Sperm of Different Sheep Breeds
The frozen sperm of 8 different breeds of sheep(2 introduced breeds,4 local breeds,and 2 culti-vated breeds)were selected for the assessment of appearance,dose,sperm motility,forward motility sperm number and sperm acrosome integrity rate.The results showed that,for the appearance,each tube of frozen sperm was in-tact and met the production standards.The dose detection value was(0.21±0.01)mL,which meetsthe national standard(≥0.18 mL).The sperm motility was(37.50%±1.70%)~(51.0%±3.70%),among which the sperm motility value of Tausset sheep was the highest,and the sperm motility value of black fur Tibetan sheep was the lowest.The number of forward spermatozoa is≥3×107,which meets the national standard.The acrosome in-tegrity rate of sperm was(71.90%±1.13%)~(81.23%±1.0%),among which the acrosome integrity rate of sperm in Tibetan(grassland)sheep was the highest,and the acrosome integrity rate of sperm in Tausset sheep was the lowest.After thawing and recovering,the frozen sperm of 8 sheep breeds all met the requirements of breeding production.The sperm motility of the introduced varieties was higher than that of the local varieties,and other indi-cators such as the number of spermatozoa and the acrosome integrity rate of forward motility were uneven.