摘要
为了解青海部分地区高效养殖环境下藏羊主要疫病的感染和流行情况,本研究通过双抗体夹心法酶联免疫吸附试验,对青海省7 个地区藏羊高效养殖场的277 份血清样品分别进行了羊传染性胸膜肺炎、羊蓝舌病、羊传染性脓疱、羊衣原体病和羊包虫病等5 种主要动物疫病的ELISA检测.结果显示,在277 份血清样本中,5 种疫病的阳性率分别为4.7%(13/277)、6.5%(18/277)、2.5%(7/277)、7.6%(21/277)和 6.9%(19/277).其中,都兰县羊传染性胸膜肺炎的阳性率最高,为14.3%(2/14);贵德县羊蓝舌病的阳性率最高,为18.2%(2/11);共和县羊传染性脓疱的阳性率最高,为9.7%(7/72);共和县羊衣原体病的阳性率最高,为19.4%(14/72);都兰县羊包虫病的阳性率最高,为14.3%(2/14).5 种疫病在乐都区和湟源县均未检出.研究结果可为当地藏羊高效养殖场主要动物疫病防控决策、疾病净化及后期无规定动物疫病区建设提供相应的科学依据.
Abstract
In order to preliminarily investigate the infection and prevalence of main diseases of Tibetan sheep in high-efficiency breeding environment in some areas of Qinghai Province,this research adopted enzyme-linked immunosorbent assay(ELISA)with double antibody sandwich method.Five major animal diseases,such as Sheep contagious pleuropneumonia,Bluetongue,Contagious ecthyma,Chlamydiosis,Hydatid disease,were detected by ELISA in 277 serum samples from Tibetan sheep farms in 6 areas of Qinghai Province.The results showed that the positive rates of five diseases were 4.7%(13/277),6.5%(18/277),2.5%(7/277),7.6%(21/277),6.9%(19/277),respectively.Dulan county had the highest positive rate of Sheep contagious pleuropneumonia(14.3%,2/14).The highestpositive rate of Bluetongue was18.2%(2/11)in Guide county.The positive rate of Contagious ecthyma was the highest in Gonghe county(9.7%,7/72).The highestpositive rate of Chlamydiosis was 19.4%(14/72)in Gonghe county.Dulan county had the highest positive rate of Hydatid disease(14.3%,2/14).None of the five diseases were detected in Ledu district and Huangyuan county.The results can provide scientific basis for animal disease prevention and control decision-making,disease purification and the construc-tion of unregulated animal epidemic areas in local Tibetan sheep high-efficiency breeding farms.
基金项目
青海省农业农村厅项目(青农函[2020]129号)
青海省质量和标准研究院项目(DB63JH-123-2023)
青海大学农牧学院项目(NKX202118)
NETL
NSTL
万方数据