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一起羊巴氏杆菌与链球菌混合感染的实验室诊断

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羊多杀性巴氏杆菌病又称为出血性败血症,是一种高度接触性传染病,其病流行会给羊场造成重大的经济损失.羊链球菌感染可引起患畜出现发热、下颌及咽喉部肿胀、纤维素性肺炎等症状,甚至导致死亡.2023 年3 月中旬,湖南省浏阳市某羊场发生疫情,部分羊群出现发热、咳嗽和呼吸困难等症状,且病死率高.为确诊病因,采集组织病料送至湖南中科基因检测公司进行病原诊断.结果发现,送检病料样品中未检测出相关病毒核酸,但分离出了羊巴氏杆菌和羊链球菌.经药敏试验发现,羊巴氏杆菌对链霉素、头孢噻呋、庆大霉素、阿莫西林、四环素、头孢他啶、头孢曲松、恩诺沙星和复方新诺明等抗菌药敏感;链球菌对阿奇霉素、头孢噻呋、磺胺甲氧嘧啶、头孢他啶和头孢曲松高度敏感.实验室诊断结果表明,该场羊群发生疫情是羊巴氏杆菌和链球菌混合感染引起的,且不同病原菌对抗菌谱存在差异,其结果为羊场疾病的治疗与防控提供了参考.
Laboratory Diagnosis of A Case of Mixed Infection of Pasteurella and Streptococcus in Sheep
Sheep pasteurellosis,also known as hemorrhagic sepsis,is a highly contagious disease which causes significant economic losses to sheep farms.The clinical symptoms caused by Streptococcus suis(S.suis)infection in sheep are mainly characterized by fever,swelling of the lower jaw and throat,fibrous pneumonia,and even death.In mid March 2023,a sheep farm in Liuyang City,Hunan Province experienced an epidemic,with some sheep ex-periencing symptoms such as fever,cough,and difficulty breathing,and a high mortality rate.To diagnose the pathogenic agents,tissue samples were collected and sent to Hunan Zhongke Gene Testing Company for pathogen diagnosis.The results showed that no relevant viral nucleic acidswere detected in the collected samples,but Pas-teurella lamblia and S.suis were successfully isolated.The drug sensitivity tests revealed that P.lamblia was sensi-tive to antibiotics such as Streptomycin,Cefotaxime,Gentamicin,Amoxicillin,Tetracycline,Ceftazidime,Ceftri-axone,Enrofloxacin,and Sulfamethoxazole;S.suis is highly sensitive to Azithromycin,Cefotaxime,Sulfamethox-azole,Ceftazidime,and Ceftriaxone.The laboratory diagnosis results indicated that the disease outbreak was caused by a mixed infection of P.lamblia and S.suis,and there are differences in the antimicrobial spectrum of dif-ferent pathogens.The results provide a reference for the treatment and prevention of sheep farm diseases.

SheepPasteurella lambliaStreptococcus suisMixed infectionLaboratory diagnosis

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长沙县农业农村局,湖南 长沙 410100

巴氏杆菌 链球菌 混合感染 实验室诊断区

2024

青海畜牧兽医杂志
青海省畜牧兽医学会 青海省畜牧兽医科学院

青海畜牧兽医杂志

影响因子:0.196
ISSN:1003-7950
年,卷(期):2024.54(2)
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