Laboratory Diagnosis of A Case of Mixed Infection of Pasteurella and Streptococcus in Sheep
Sheep pasteurellosis,also known as hemorrhagic sepsis,is a highly contagious disease which causes significant economic losses to sheep farms.The clinical symptoms caused by Streptococcus suis(S.suis)infection in sheep are mainly characterized by fever,swelling of the lower jaw and throat,fibrous pneumonia,and even death.In mid March 2023,a sheep farm in Liuyang City,Hunan Province experienced an epidemic,with some sheep ex-periencing symptoms such as fever,cough,and difficulty breathing,and a high mortality rate.To diagnose the pathogenic agents,tissue samples were collected and sent to Hunan Zhongke Gene Testing Company for pathogen diagnosis.The results showed that no relevant viral nucleic acidswere detected in the collected samples,but Pas-teurella lamblia and S.suis were successfully isolated.The drug sensitivity tests revealed that P.lamblia was sensi-tive to antibiotics such as Streptomycin,Cefotaxime,Gentamicin,Amoxicillin,Tetracycline,Ceftazidime,Ceftri-axone,Enrofloxacin,and Sulfamethoxazole;S.suis is highly sensitive to Azithromycin,Cefotaxime,Sulfamethox-azole,Ceftazidime,and Ceftriaxone.The laboratory diagnosis results indicated that the disease outbreak was caused by a mixed infection of P.lamblia and S.suis,and there are differences in the antimicrobial spectrum of dif-ferent pathogens.The results provide a reference for the treatment and prevention of sheep farm diseases.