首页|酶法制备牦牛骨肽及抗氧化活性研究

酶法制备牦牛骨肽及抗氧化活性研究

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为研究牦牛骨肽的提取率和抗氧化活性,本试验以青海牦牛骨为原料,分析了不同蛋白酶对牦牛骨肽提取率和体外抗氧化活性的影响.结果显示,不同蛋白酶对牦牛骨提取率和抗氧化活性均有一定影响.碱性蛋白酶牦牛骨肽提取率为14.67 mg/mL,DPPH自由基清除率为(73.19±2.18)%,·OH清除率为(64.35±3.25)%,还原力为0.192±0.045,均显著高于其余蛋白酶(P<0.05).因此,碱性蛋白酶制备的牦牛骨肽具有提取率高和抗氧化活性好的优势,为牦牛骨肽功能性产品的开发提供了数据参考.
Preparation of Yak Bone Peptide and Antioxidant Activity by Enzymatic Method
In order to study the extraction rate and antioxidant activity of yak bone peptide,the effects of dif-ferent proteases on the extraction rate and antioxidant activity of yak bone peptide in vitro were analyzed.The re-sults showed that different proteases had certain effects on the extraction rate and antioxidant activity of yak bones.The extraction rate of alkaline protease yak bone peptide was 14.67 mg/mL,and the DPPH free radical scavenging rate was(73.19±2.18)%,The clearance rate of·OH was(64.35±3.25)%,and the reducing power was 0.192±0.045,which were significantly higher than those of other proteases(P<0.05).Therefore,the prepara-tion of yak bone peptide by alkaline protease has the advantages of high extraction rate and good antioxidant activi-ty,which provides a data reference for the development of functional products of yak bone peptide.

Yak bone peptideEnzymatic lysis methodFree radicaIn vitro antioxidant activity

林青、吴海玥、胡蓉、刘云娜、任海佳、胡博、闫忠心

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青海大学,青海西宁 810016

青海省牦牛工程技术研究中心,青海西宁 810016

牦牛骨肽 酶解法 自由基 体外抗氧化活性

西宁市科技计划项目青海省中央引导地方资金项目

2022-Y-112023ZY027

2024

青海畜牧兽医杂志
青海省畜牧兽医学会 青海省畜牧兽医科学院

青海畜牧兽医杂志

影响因子:0.196
ISSN:1003-7950
年,卷(期):2024.54(4)