首页|基于巨型住肉孢子虫SPFH-SG蛋白ELISA方法的建立

基于巨型住肉孢子虫SPFH-SG蛋白ELISA方法的建立

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本试验以藏绵羊食道肌巨型住肉孢子虫(Sarcocystis gigantea)SPFH-SG蛋白为研究对象,通过基因克隆及蛋白表达,建立了一种特异性、重复性均良好的间接ELISA检测方法,为藏羊住肉孢子虫病的批量临床检测提供技术方案.
Establishment of an ELISA Method Based on the SPFH-SG Protein of Sarcocystis Gigantea
Sarcocystosis is a zoonotic parasitic disease caused by parasitic protozoa Sarcocystis living in host cells,which seriously affects the healthy development of livestock and human food safety.Currently,there are rela-tively few studies on diagnostic methods for this disease,especially on prenatal diagnostic techniques.This study focuses on the SPFH-SG protein of Sarcocystis gigantea from the esophageal muscle of Tibetan sheep.Through gene cloning and protein expression,a specific and reproducible indirect ELISA method has been established,pro-viding a scientific basis and technical solution for the ante-mortem detection of sarcocystosis in Tibetan sheep.

Sarcocystis giganteaELISASPFH-SG proteinTibetan sheep

郭良婷、康明

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青海大学农牧学院,青海 西宁 810016

巨型住肉孢子虫 酶联免疫吸附实验 SPFH-SG蛋白 藏羊

青海省科技厅项目国家自然科学基金

2021-ZJ-73532060806

2024

青海畜牧兽医杂志
青海省畜牧兽医学会 青海省畜牧兽医科学院

青海畜牧兽医杂志

影响因子:0.196
ISSN:1003-7950
年,卷(期):2024.54(5)