Effects of Yersinia Pestis Lipopolysaccharide on the Organs of Mice
To investigate the effects of different doses of lipopolysaccharide(LPS)extracted from Yersinia pestis on the general state and organs of mice,so as to provide experimental data for studying the toxic mechanism of LPS on humans and theoretical basis for clinical diagnosis of early endotoxin infection.Yersinia pestis was cultured by conventional methods,and LPS was extracted by kit method.The components of the extracted LPS were identified and SDS-PAGE electrophoresis was performed.The mice were intraperitoneally injected with LPS in line with the experimental requirements,and the phenotypic changes of the mice were monitor.The mice were sacrificed,dis-sected and organs are weighted and the degree of organ damage induced by LPS was counted.The results of this ex-periment showed that the purity of LPS samples was 91%,and the electrophoretic bands distributed between 10 and 17 Ku.Intraperitoneal injection of LPS of Yersinia pestis could cause mice to show symptoms such as decreased ac-tivity,curled up,weakness,fluff and lethargy,and the liver and spleen enlarged and congested.With the increase of LPS injection dose,the degree of organ damage in the experimental group also aggravated.But with LPS injected does 450 μg/pcs,the wet weight of liver was lower than that of 350 μg/pcs and 250 μg/pcs,and the wet weight of spleen was lower than that of 350 μg/pcs.A certain dose of LPS can cause organ damage,oxidative stress and immune system overactivation in experimental animals.With the increase of LPS injection dose,the degree of inju-ry aggravated.
万方数据
维普
