Comparative Experiment on Frozen Semen Quality of Different Breeds of Bulls in High-altitude Areas
This experiment was conducted in the Guoluo area with an average altitude of 4 200 meters.Five breeds of male cattle,including wild yak,Datong yak,Chaidamu yellow cattle,Holstein,and Juanshan cattle,were subjected to frozen semen in thin tubes after thawing in a 39℃water bath.The semen was then placed at temperatures of 26℃and 36.5℃,and sperm viability was measured every 2 hours,the vitality index of sperm was evaluated based on the degree of change in sperm vitality.The results showed that the frozen sperm of the same breed of cattle were thawed under the same conditions and stored at 26℃and 36.5℃,and there was no signifi-cant difference in sperm vitality rate within 2 hours(P>0.05).However,after 4 hours,the sperm vitality rate stored at 26℃was significantly higher than that stored at 36.5℃,and the difference was significant(P<0.05).At 26℃storage,the sperm viability of 5 breeds of cattle gradually decreased with the prolongation of time.Within 6 hours,the difference in sperm viability between Datong yak and wild yak was slightly lower than that of Chaidamu cattle,and significantly lower than that of Holstein cattle and Juanshan cattle(P<0.01).The experiment showed that the changes in sperm viability of different breeds from low to high at the same time were:wild yak>Datong yak>Chaidamu yellow cattle>Holstein>Juanshan cattle.
High altitudeDifferent breeds of bullsSperm viabilitySurvival time
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